益气化瘀解毒方对人肝癌裸鼠HepG2移植瘤MVD、HIF1a、VEGF/KDR表达的影响  

Effect of Prescription of Strengthening Qi and Clearing Poison and Toxin on Expressions of MVD,HIF1a and VEGF/KDR from Hepg2 Hepatocellular Carcinoma Nude Mice Transplantation Tumor

作　　者：曾普华[1] 郜文辉[2] 潘敏求[1] 蒋益兰[1] 李勇敏[1] 李跃辉[1] 唐珍[1] 

机构地区：[1]湖南省中医药研究院附属医院肿瘤科,湖南长沙410006 [2]湖南中医药大学中医学院,湖南长沙410208

出　　处：《中华中医药学刊》2014年第7期1563-1565,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81001522);湖南省自然科学基金项目(13JJ4138);首届中国中西医结合学会-金港榄香烯中医药肿瘤研究基金项目(2010-F10)

摘　　要：目的:观察益气化瘀解毒方对人肝癌裸鼠HepG2移植瘤MVD、HIF1a、VEGF/KDR表达的影响。方法:建立人肝癌移植瘤模型,分为7组,分别以益气化瘀解毒方、黄芪、莪术、重楼、壁虎、顺铂药物干预21 d后,取瘤组织行免疫组化检测。结果:①对MVD的影响:全方组与除顺铂外的其它组比较有统计学意义(P<0.05);除黄芪、莪术外的其它组与空白组比较有统计学意义(P<0.05)。②对HIF1a的影响:全方组与除黄芪外的其它组比较有统计学意义(P<0.05);各组与空白组比较有统计学意义(P<0.05)。③对VEGF表达的影响:全方组与除顺铂外的其它组比较均有统计学意义(P<0.05);莪术组与空白组比较无统计学意义(P>0.05)。④对KDR表达的影响:全方组与其它各组比较均有统计学意义(P<0.05);黄芪组与空白组比较无统计学意义(P>0.05)。结论:益气化瘀解毒方能明显抑制人肝癌裸鼠移植瘤MVD、HIF1a、VEGF和KDR的表达而发挥抗血管生成作用;各单味药对其作用各有侧重,但作为整方的有机组成从不同环节发挥了协同作用。Objective：To observe the effects of Prescription of Strengthening Qi and clearing stasis and poison（SQCSP）of the expressions of MVD,HIF1a and VEGF/KDR from HepG2 hepatocellular carcinoma nude mice transplantation tumor（HepG2 Tumor）.Methods：HepG2 tumor was divided into 7 groups,respectively intervened by SQCSP,astragalus root,rhizoma zedoariae,paris polyphylla,gecko and cisplatin.After 21 d of drug intervention,immunohistochemical detection was carried out in tumor tissue.Results：（1） The effect on MVD：SQCSP group compared with the others except the cisplatin group was statistically significant（P〈0.05）.In addition to the radix astragali,rhizoma zedoariae in other groups compared with that of blank group was statistically significant（P 〈 0.05）.（2） The effect on HIF1a：SQCSP group compared with others except astragalus membranaceus group was statistically significant（P 〈 0.05）.Each group compared with the blank group was statistically significant（P〈0.05）.（3） The expression of VEGF：the influence of SQCSP group compared with the others except the cisplatin group had statistical significance（P〈 0.05）.Rhizoma zedoariae group compared with the blank group had no statistical significance（P〉0.05）.（4） The expression of KDR：the influence of SQCSP group compared with other groups had statistical significance（P〈0.05）.Astragalus membranaceus group compared with the blank group had no statistical significance（P〉0.05）.Conclusion：SQCSP can inhibit the expressions of MVD,HIF1a and VEGF/KDR from HepG2 hepatocellular carcinoma nude mice transplantation tumor and enhance anti-angiogenesis.Each single herb has own effect particularly,but as the whole party,they play a synergy effect.

关 键 词：益气化瘀解毒方 人肝癌裸鼠HepG2移植瘤 MVD HIF1a VEGF/KDR 

分 类 号：R285.5[医药卫生—中药学]