姜黄素对直肠癌细胞放疗增敏作用的研究  

Radiosensitive Effect of Curcumin for Rectal Cancer Cells

作　　者：陆新建 裘建明 杨关根 王幸 封巍 沈忠 

机构地区：[1]兴化市人民医院,江苏兴化225700

出　　处：《中华中医药学刊》2014年第7期1588-1590,I0003,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学青年基金项目(81101876/H1617);浙江省自然科学基金项目(Y2110782);浙江省科技厅公益技术研究社会发展项目(2012C33SA100045)

摘　　要：目的:探讨姜黄素(curcumin)对人直肠癌细胞放射增敏作用。方法:体外实验:将HR8348细胞,分成四组:对照组(给予DMSO液);姜黄素组(给予2.5μM姜黄素);照射组(单纯X线照射);联合组(给予2.5μM姜黄素,联合X线照射)。分别采用MTT和流式细胞仪检测细胞的生长抑制情况和凋亡情况;体内实验:建立裸鼠移植肿瘤模型,单独及联合应用姜黄素和放射处理裸鼠移植瘤,通过观察肿瘤生长变化及瘤体凋亡情况并进行统计分析。结果:姜黄素对人直肠癌HR8348细胞增殖有明显的抑制作用,明显提高放射对裸鼠移植瘤的杀伤作用。结论:姜黄素对人直肠癌HR8348细胞有放射增敏作用,姜黄素在直肠癌治疗中可能成为一种有希望的放射增敏剂。Objective：To investigate the sensitization of low concentration of curcumin on radiotherapy of rectal cancer celsl.Methods：HR-8348 cells were randomly divided into curcumin group,radiotherapy group,curcumin plus radiotherapy group and control group.Cell growth inhibition was assayed by MTT method.The apoptotic rates were detected by FCM with Annexin V/PI staining.And then 5-week-old BALB/c nude mice bearing the rectal cancer cell in the footpad of the right were randomly divided into the same four groups.The size of the tumor was measured every 3 days.After15 days,apoptosis was detected with TUNEL.Results：Low concentration of curcumin could enhance the effect of radiation both in growth inhibition and apoptosis of rectal cancer cells.Conclusion：Curcumin can enhance the sensitivity of radiotherapy.It may become a potential radiosensitizing agent in the future.

关 键 词：姜黄素 直肠癌 细胞凋亡 放射增敏 

分 类 号：R755.3[医药卫生—皮肤病学与性病学]