HPLC法测定朝鲜淫羊藿及市售淫羊藿中淫羊藿苷等四种成分研究  

Determination of Four Components of Korean Epimedium and Commercially Available Epimedium

作　　者：陈婷[1] 许亮[1] 杨燕云[1] 李先宽[1] 丁璞[1] 宋新[1] 王冰[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中华中医药学刊》2014年第7期1621-1623,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：中华人民共和国药典2015版显微鉴别项目(YD201501);辽宁省科技厅项目(20111133);辽宁中医药大学杰出青年基金项目(20121228)

摘　　要：目的：建立同时测定淫羊藿样品中淫羊藿苷、朝藿定A、朝藿定B、朝藿定C含量的HPLC方法,为完善淫羊藿市售药材及饮片的质量评价提供参考。方法：采用高效液相色谱法,以Agilent C18（4.6 mm×250 mm,5μm）为色谱柱,乙腈-水为流动相进行梯度洗脱,体积流量1.0 mL/min,柱温25℃,检测波长270 nm。结果：淫羊藿苷、朝藿定A、朝藿定B、朝藿定C分别在0.0800～1.60μg、0.0175～0.350μg、0.0775～1.55μg、0.233～4.66μg（r≧0.9995）范围内呈良好的线性关系,平均加样回收率分别为96.5%、97.4%、97.9%、97.4%,RSD分别为1.3%、1.5%、1.0%、1.8%。结论：所建立的方法简便、准确、重复性好,可以为朝鲜淫羊藿及市售淫羊藿质量评价提供参考。Objective：To establish an HPLC method for simultaneously determining the icariin,Epimendin A,Epimendin B and Epimendin C contents in Epimedium koreanum Nakai and to provide the quality evaluation for the commercially available Epimedium koreanum Nakai herbs.Method：Agilent C18（4.6 mm ×250 mm,5 μm） column in an oven at 25 ℃ was needed with the mobile phase including acetonitrile-water.The flow rate was 1.0 mL/min with a UV detector at 270 nm.Result：The linear range of icariin was 0.0800 ～ 1.60 mg/mL（r = 0.9999） and the average recovery was 96.5%,RSD 1.3%.The linear range of Epimendin A was 0.0175 ～ 0.350 mg/mL（r = 0.9998） and the average recovery was 97.4%,RSD 1.0%.The linear range of Epimendin B was 0.0775 ～ 1.55 mg/mL（r = 0.9999） and the average recovery was 97.9%,RSD 1.0%.The linear range of Epimendin C was 0.233 ～ 4.66 mg/mL（r = 0.9995） and the average recovery was 97.4%,RSD 1.8%.Conclusion：The method appeared to be simple,accurate and reliable,and can provide the basis for Korean Epimedium＇s quality evaluation.

关 键 词：淫羊藿 高效液相色谱 淫羊藿苷 朝藿定A 朝藿定B 朝藿定C 

分 类 号：R284.1[医药卫生—中药学]