石香薷总黄酮的大孔树脂动态吸附纯化工艺研究  

On the Purification of Total Flavonoids from Mosla Chinensis by Macroporous Resin

作　　者：施群[1] 施淑琴[2] 俞文英[3] 钟宇森[3] 

机构地区：[1]金华市中心医院,浙江金华321000 [2]金华职业技术学院,浙江金华321007 [3]浙江省医学科学院,浙江杭州310013

出　　处：《中华中医药学刊》2014年第7期1655-1658,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金项目(Q12H280035;Y2111058);浙江省科技厅项目(2010R410055)

摘　　要：目的:确定HPD300大孔吸附树脂吸附纯化石香薷总黄酮的工艺条件。方法:采用静态吸附和动态吸附分离法,确定石香薷总黄酮的纯化工艺条件。结果:HPD300大孔树脂对石香薷总黄酮有良好的吸附分离性能,其动态吸附分离工艺条件为:石香薷总黄酮上样液(pH=3.0)浓度为5.5 mg/mL,最大吸附量为41.58 mg/g,吸附流速为2.0 mL/min,以8倍柱体积60%乙醇(pH=8.0)洗脱,树脂可重复使用4次。此工艺下石香薷总黄酮的回收率达89.12%,纯度为90.77%。结论:采用HPD300大孔树脂吸附分离石香薷总黄酮简便有效,回收率高。Objective：To investigate the purification of total flavonoids extracted from Mosla chinensis by HPD300macroporous resin.Method：The dynamic conditions of adsorption and separation were analyzed with HPD300 macroporous resin.The flavonoids adsorption capacity was used as the evaluation criteria.Result：HPD300 had the good performance for adsorbing and separating total flavonoids from M.chinensis under the following technological conditions：the concentration of the flavonoids sample extract（pH = 3.0） was 5.5 mg/mL.The maximum adsorbing capacity was 41.58 mg/g;the current velocity was 2.0 mL/min;the elutingreagent was 60% ethanol（pH = 8.0,and eight times as the volume of the resin）.And HPD300 resin could be used four times.Under these conditions,the experimental yield was 89.12% and the contents of total flavonoids in the extract were detected to be 90.77%.Conclusion：It is a simple and efficient method to separate total flavonoids from M.chinensis and the total flavone recovery rate is high.

关 键 词：石香薷 总黄酮 大孔树脂 

分 类 号：R284[医药卫生—中药学]