甘丙肽在成年雄性小鼠睾丸和附睾中的表达定位研究  

Expression of galanin in the testis and epididymis of adult mouse

作　　者：石翠格[1] 王丽丽[1] 李慧[2] 武斌[1] 王介东[1] 徐志卿[2] 张树成[1] 

机构地区：[1]国家人口计生委科学技术研究所,北京100081 [2]首都医科大学神经生物学系

出　　处：《中国计划生育学杂志》2014年第7期448-450,共3页Chinese Journal of Family Planning

基　　金：国家重点基础研究发展计划(2010CB530403)

摘　　要：目的:研究甘丙肽在成年雄性小鼠睾丸和附睾中的表达和定位,探讨甘丙肽在雄性生殖器官中的作用。方法:采用免疫组化法检测甘丙肽在睾丸和附睾组织中的表达。结果:甘丙肽在成年小鼠睾丸和附睾组织中均有表达。睾丸组织间质细胞、支持细胞和各级生精细胞呈阳性染色,主要表达于胞膜及胞质;附睾管高柱状细胞、低柱状细胞的胞膜及胞质呈阳性染色,且甘丙肽在附睾体和尾部表达较强,在头部远段表达较弱。结论:甘丙肽在成熟小鼠睾丸和附睾组织中均有表达,其生理功能尚待阐明。Objective： To study the expression and localization of galanin in testis and epididymis of adult mice, and to explore the role of galanin in male reproductive organs in mice. Methods： The expressions of galanin were detected in both testis and epididymis by immunohistochemical staining. Results： Galanin was expressed in testis and epididymis of adult mice. In the testis,galanin immunoreactive particles were found in interstitial ceils, sertoli cells and spermatogenic cells, and mainly in the membrane and cytoplasm. And in the epididymis, galanin was presented in epididymal duct tall columnar cells, low columnar cell membrane and cytoplasm. The galanin expression in the epididymis body and tail were higher than that of the epididymis head. Conclusion： Galanin is presented in testis and epididymis of the mature mouse and its physiological function remains to be elucidated.

关 键 词：甘丙肽 睾丸 附睾 生精细胞 间质细胞 

分 类 号：R339.21[医药卫生—人体生理学]