脊髓烟酰胺腺嘌呤二核苷酸磷酸氧化酶在大鼠糖尿病神经痛维持中的作用  

Role of nicotinamide adenine dinucleotide phosphate oxidase in spinal cord in maintenance of diabetic neuropathic pain in rats

作　　者：黄晓雷[1] 李晓芸[2] 吴铭广[1] 文亚杰[1] 齐晓非[1] 胡薇[1] 李元涛[1] 

机构地区：[1]南方医科大学附属深圳妇幼保健院麻醉科,518028 [2]中山大学附属第三医院麻醉科

出　　处：《中华麻醉学杂志》2014年第4期430-432,共3页Chinese Journal of Anesthesiology

摘　　要：目的 评价脊髓烟酰胺腺嘌呤二核苷酸磷酸氧化酶（NADPH氧化酶）在大鼠糖尿病神经痛维持中的作用.方法 清洁级雄性SD大鼠,2月龄,体重180 ～ 220 g,采用腹腔注射1％链脲佐菌素（STZ）的方法制备糖尿病模型,注射STZ后72 h血糖＞16.7 mmol/L的大鼠作为糖尿病大鼠.采用随机数字表法,将20只糖尿病大鼠分为2组（n=10）：糖尿病神经痛组（DN组）和NADPH氧化酶特异性抑制剂香荚兰乙酮组（A组）,另取10只大鼠为正常对照组（C组）.A组于注射STZ后28 d腹腔注射香荚兰乙酮5 mg/kg,1次/d,连续7d.分别于注射STZ前（T1）、注射STZ后7、14、21、28、35 d（T2-6）时测定机械缩足反应阈（PWT）.于T6时PWT测定结束后,处死大鼠,取L4,5节段脊髓组织,检测NADPH氧化酶亚基gp91phox和p47phox的表达、MDA含量及SOD活性.结果 与C组比较,DN组和A组T3-5时PWT降低,脊髓gp91phpx和p47phox的表达上调,MDA含量升高,SOD活性降低（P＜0.05）;与DN组比较,A组T6时PWT升高,脊髓gp91phox和p47phox的表达下调,MDA含量降低,SOD活性升高（P＜0.05）.结论 脊髓NADPH氧化酶参与了大鼠糖尿病神经痛的维持.Objective To evaluate the role of nicotinamide adenine dinucleotide phosphate oxidase （NADPH oxidase） in the spinal cord in the maintenance of diabetic neuropathic pain in rats.Methods Pathogenfree male Sprague-Dawley rats,aged 2 months,weighing 180-220 g,were used in the study.Diabetes mellitus was induced by intraperitoneal streptozotocin （STZ） 60mg/kg and confirmed by blood glucose 〉 16.7 mmol/L at 72 h after STZ injection.Twenty diabetic rats were randomly allocated to diabetic neuropathic pain group （DN group,n =10） and apocynin （specific NADPH oxidase inhibitor） group （A group,n =10）.Another 10 agematched normal rats served as control group （C group,n =10）.Twenty-eight days after STZ injection,apyconin 5 mg/kg was injected intraperitoneally once a day for 7 consecutive days in A group.Paw withdrawal threshold to yon Frey filament stimulation （PWT） was measured before STZ injection （T1） and at 7,14,21,28 and 35 days after STZ injection （T2-6）.The rats were sacrificed after PWT was measured at T6 and L4.5 segments of the spinal cord were removed for determination of NADPH oxidase subunits gp91phox and p47phox expression,MAD content and SOD activity.Results Compared with C group,PWT was significantly decreased at T3-5,gp91phox and p47phox expression was up-regulated,MAD content was increased,and SOD activity was decreased in DN and A groups.Compared with DN group,PWT was significantly increased at T6,gp91phox and p47phox expression was downregulated,MAD content was decreased,and SOD activity was increased in A group.Conclusion NADPH oxidase in the spinal cord is involved in the maintenance of diabetic neuropathic pain in rats.

关 键 词：氧化还原酶类 糖尿病 神经痛 脊髓 

分 类 号：R587.2[医药卫生—内分泌]