HPLC同时测定杜仲叶中绿原酸和芦丁方法的建立及在杂种后代选择中的应用  

Simultaneous Determination of Chlonogenic Acid and Rutin in the Leaves of Eucommia ulmoides by HPLC and Its Use in the Selection of Hybrids

作　　者：魏军坤 刘闵豪 杨秀平[1] 李煜[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2014年第5期129-132,共4页Journal of Northwest Forestry University

基　　金：林业公益性行业科研专项(201204605)

摘　　要：建立利用高效液相法（HPLC）同时测定杜仲叶中绿原酸和芦丁的方法。色谱柱为Aglient ZORBAX SB-C18（4.6mm×250mm,5μm）,流动相组成为甲醇-乙酸-水（体积比为50∶1∶49）,检验波长为340nm,流量为1mL/min,检测温度为32℃。在该色谱条件下,绿原酸和芦丁分别在0.060 0～2.000 0μg/mL和0.001 8～0.090 0μg/mL范围内线性关系良好,相关系数分别为0.999 7和0.999 3。利用此方法对杜仲杂交后代中41株生长性状表现优良的个体进行了叶片中绿原酸和芦丁含量的测定,为杜仲优良无性系的选择提供依据。A convenient high performance liquid chromatographic（HPLC）method has been established for the simultaneous determination of chlonogenic acid and rutin in the leaves of Eucommia ulmoides.Two components were saperated by the column Aglient ZORBAX SB-C18（4.6mm×250mm,5μm）with a mobile phase of methanol-acetic acid-water（50∶1∶49,V/V）at a flow rate of 1mL/min,detection wavelength of 240nm and column temperature of 32℃.The chlonogenic and rutine each showed good liner relationship in a range of 0.060 0μg/mL～2.000 0μg/mL and 0.001 8μg/mL～0.090 0μg/mL,respectively,with the correlation cofficients of 0.999 7and 0.999 3.The two components in the leaves of 41fine individual clones were determined using the method above to provide basis for selection of superior clones.

关 键 词：杜仲 HPLC 绿原酸 芦丁 优良单株 

分 类 号：S781.41[农业科学—木材科学与技术]