稀酸预处理铜藻制备生物乙醇工艺  

Optimization of Sargassum horneri Pretreatment Process Using Diluted Acid for Bioethanol

作　　者：蒋媛媛[1] 包海军[2] 曾淦宁[1] 艾宁[2] 王铁杆[3] 张茹霞[1] 吴勇[1] 

机构地区：[1]浙江工业大学海洋学院,浙江杭州310014 [2]浙江工业大学化学工程学院,浙江杭州310014 [3]浙江省海洋水产养殖研究所,浙江温州325000

出　　处：《环境科学研究》2014年第7期804-812,共9页Research of Environmental Sciences

基　　金：浙江省自然科学基金项目(LY12D06004);浙江省科技厅公益项目(2013C33005)

摘　　要：选取暖温带海洋生态环境生物修复的首选物种——铜藻(Sargassum horneri)为原料进行生物乙醇的制备,以稀硫酸水解后的还原糖收率为响应值,考察水解温度、液固比、水解时间和w(H2SO4)等参数对水解效率的影响.为优化稀酸水解铜藻预处理的工艺条件,在单因素试验的基础上,利用Box-Benhnken中心组合设计法和响应面分析法,建立稀酸预处理工艺参数的回归模型,并与酶水解及发酵相结合验证了铜藻稀酸预处理效果.结果表明:①稀酸水解铜藻的最优工艺参数.水解温度为120℃,液固比为20∶1,水解时间为2.00 h,w(H2SO4)为4.50%.②稀酸水解铜藻过程中各影响因素之间存在交互作用,水解时间和w(H2SO4)的非线性作用显著.③对经最佳稀酸预处理工艺处理后的铜藻粉进行酶水解,其还原糖收率为44.05%,是未预处理下的8.14倍,并且后续进行发酵后,乙醇产率达7.80%,是未预处理下的2.00倍.表明铜藻是一种潜在的生物乙醇原料,稀酸预处理方法对铜藻生物乙醇的制备行之有效.Ethanol is an increasingly common,environmentally-friendly renewable fuel. This study investigated bioethanol production from Sargassum horneri,a preferred species to repair warm temperature marine ecological environments. The effects of hydrolysis pretreatment including hydrolyzing temperature,liquid-solid ratio,time and concentration of sulfuric acid on hydrolysis efficiency were studied on the basis of the hydrolysis rate of sugar reduction. A parametric regression model of S. horneri pretreatment process was established according to Box-Behnken design and response surface methodology,and the pretreatment performance of diluted acid was investigated by combining with enzyme hydrolysis and fermentation. The results showed that the optimal values of the parameters were hydrolysis temperature of 120℃,liquid-solid ratio of 20∶ 1,hydrolysis time of 2. 00 h and acid concentration of 4. 50%. The interactions of different factors could not be ignored; the nonlinear effect between hydrolysis time and the concentration of sulfuric acid was significant. The recovery of reducing sugar and bio-ethanol was 44. 05% and 7. 80%,respectively,which was eight and two times higher than the untreated S. horneri,when the species was preprocessed under the optimum acid hydrolysis. It was concluded that S. horneri is a potential bioethanol source species,and dilute sulfuric acid pretreatment is an effective pretreatment method to produce bioethanol.

关 键 词：铜藻 稀酸水解 还原糖收率 响应面法 生物乙醇 

分 类 号：X705[环境科学与工程—环境工程]