曲尼司特对膀胱出口部分梗阻后膀胱功能的保护作用  

The protection of tranilast on bladder function after partial bladder outlet obstruction

作　　者：王博[1] 姜晓晓[1] 彭云鹏[1] 庄岩[1] 孙晓青[1] 朱海涛[1] 

机构地区：[1]徐州医学院,江苏徐州221002

出　　处：《国际泌尿系统杂志》2014年第4期518-522,共5页International Journal of Urology and Nephrology

基　　金：徐州市科技发展基金（XF11C066）

摘　　要：目的 探讨曲尼司特对膀胱出口部分梗阻（BOO）模型大鼠膀胱功能的保护作用及其作用机制.方法 健康雌性Wistar大鼠30只随机分为空白对照组、BOO组和曲尼司特组.BOO组和曲尼司特组行尿道远端梗阻方式制备膀胱出口部分梗阻模型,空白对照组采用假手术方式处理.曲尼司特组术前1天及以后每天给予曲尼司特[400mg·（kg·d）-1]灌胃治疗,BOO组和空白对照组每天给予等体积的生理盐水灌胃,持续4周.4周后采用尿流动力仪检测膀胱容量及最大膀胱压等指标,评估膀胱的功能;测量膀胱的厚度和重量;作HE染色观察膀胱肌层增生程度;应用免疫组织化学方法检测膀胱组织转化生长因子-β1（TGF-β1）的表达.结果 与空白对照组相比,BOO组尿流动力学表现最大压力（cmiH2O）与容量（mL）明显升高（62.71±9.71 vs 31.10±4.53,P＜0.05,0.72±0.13 vs 0.30±0.06,P＜0.05）,术后膀胱厚度（μm）、重量（mg）增加（分别为1038.5±148.4 vs 727.1 ±74.9,P＜0.05,182.3±23.5 vs 119.0±36.0,P＜0.05）,HE染色膀胱增生显著,膀胱纤维化程度未见明显增高.TGF-β1的表达增高（0.37±0.06 vs0.17±0.04,P＜0.05）.与BOO组相比,曲尼司特组尿流动力学表现最大压力（cmH2O）和容量（mL）减弱（48.48±4.72 vs 62.71±9.71,P＜0.05,0.51 ±0.06 vs 0.72±0.13,P＜0.05）,膀胱厚度（μm）、重量（mg）减少（分别为886.3±96.4 vs 1038.5 ±148.4,P＜0.05,155.1±16.3 vs 182.3±23.5,P＜0.05）,HE染色显示膀胱增生减弱,TGF-β1的表达降低（0.29±0.06vs0.37±0.06,P＜0.05）.结论 曲尼司特对膀胱收缩功能有显著的保护作用,可能是通过降低TGF-β1表达改善膀胱功能.Objectives To explore the intervention of tranilast on bladder function after bladder outlet obstruction in rats,and discuss mechanism of action.Methods 30 healthy 90-day-old Wistar rats were equally divided into three groups at random：sham operation group,bladder outlet obstruction （BOO） group and tranilast-treated BOO group.Tranilast was filled into the stomach one day before the operation and ever day after for 4 weeks.The same volume of water was poured down into the stomach of rats in the group of the sham operation and BOO every day.After 4 weeks rats in every group was sedated and cystometry was assayed.Urodynamic parameters were bladder capacity and maximum voiding pressure.After cystometry the bladder was weighed and the thickness of the bladder was measured.Histological changes of bladder were observed by HE.Immunohistochemical method was to detect bladder tissue expression of TGF-β 1.Results Compared with sham operation group,obstructed group showed significantly high maximal voiding pressure and capacity （62.71 ±9.71 vs 31.10 ±4.53 P ＜0.05,0.72 ±0.13 vs O.30 ± 0.06 P ＜ 0.05） resulting in bladder thickness and weight increased significantly（1038.5 ± 148.4 vs 727.1 ± 74.9 P ＜ 0.05,182.3 ± 23.5 vs 119.0 ± 36.0 P ＜ 0.05）.HE staining was consistent with muscle hypertrophy in the BOO group.Bladder tissue expression of TGF-β1 rised（0.37 ± 0.06vs0.17 ± 0.04 P ＜ 0.05）.Compared with BOO group,tranilast-treated BOO group had the low maximal voiding pressure and capacity （48.48 ± 4.72 vs 62.71 ± 9.71 P ＜ 0.05,0.51 ± 0.06 vs 0.72 ± 0.13 P ＜ 0.05）with the bladder thickness and weight decreasing（886.3 ± 96.4 vs 1038.5 ± 148.4 P ＜ 0.05,155.1 ± 16.3 vs 182.3 ± 23.5 P ＜ 0.05）.HE staining showed an abvious improvement of muscle hypertrophy in treated group.Bladder tissue expression of TGF-β1 declined （0.29 ± 0.06 vs0.37± 0.06 P ＜ 0.05）.Conclusions Tranilast has obvious protective effect of bladder contraction function,red-ucing the TGF-beta 1 expre

关 键 词：膀胱疾病 疾病模型 动物 大鼠 

分 类 号：R694[医药卫生—泌尿科学]