副鸡禽杆菌3个血清型参考菌株生长曲线的测定和比较  

Determination and Comparative Analysis of Growth Curves of Three Reference Strains of Avibacterium paragallinarum Serotype A,B and C

作　　者：姚东璧[1,2] 李复煌[3] 谢三磊[4] 李桂萍[1] 徐福州[1] 房慧伶[2] 孙惠玲[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京畜禽疫病防控技术北京市重点实验室,北京100097 [2]广西大学动物科学技术学院,广西南宁530004 [3]北京市畜牧兽医总站,北京100107 [4]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《动物医学进展》2014年第7期43-46,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30871867);北京市新世纪百千万人才工程项目

摘　　要：为探索副鸡禽杆菌生长特性,比较不同血清型菌株生长特性的差异,采用分光光度法和菌落计数法分别测定副鸡禽杆菌血清A型(221株)、血清B型(0222株)、血清C型(Modesto株)3个参考菌株的生长曲线,对两种方法测定的生长曲线进行比较。结果表明,两种方法绘制的生长曲线差异较大,对数生长期和稳定期菌液OD 600nm值与活菌数对数之间呈现一元二次多项式回归关系。另外,3个血清型菌株的生长稳定期都比较短暂,各型菌株达到生长高峰的时间和活菌数都有差异。该试验为副鸡禽杆菌生物学特性的比较研究及疫苗的研制奠定了基础。To explore and compare the growth changes and growth characteristics of different serotype of Avibacterium paragallinarum,growth curves of three reference strains of serotype A（221strain）,B（0222strain）and C（Modesto strain）were determined by spectrophotometry and colony count methods under the same culture conditions.Then two methods were compared.Results showed that the growth curves made by two methods were different.OD 600 nm and the logarithm of colony count in logarithmic phases and stationary phase showed a quadratic polynomial regression.In addition,stationary phases of three serotypes were short and differences were observed on reaching the growth peak and viable colony counts in three serotypes.This experiment laid a foundation for the further comparative study on the biological characteristics and vaccine development of Avibacterium paragallinarum.

关 键 词：副鸡禽杆菌 生长曲线 生长特性 

分 类 号：S852.613[农业科学—基础兽医学]