鸭疫里默菌分离鉴定及药敏试验  

Isolation,Identification and Drug Susceptibility Test of Riemerella anatipestifer

作　　者：吴彩艳[1] 邹秀娟 戚南山[1] 廖申权[1] 李娟[1] 温列娜[1] 吕敏娜[1] 孙铭飞[1] 

机构地区：[1]广东省农业科学院动物卫生研究所,广东广州510640 [2]广东天农食品有限公司,广东清远511827

出　　处：《动物医学进展》2014年第7期69-72,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31201902);国家自然科学基金项目(31302086);珠江科技新星项目(2012J2200059);广东省农业科学院院长基金项目(201413);广东省农业攻关项目(2012A020100001)

摘　　要：从疑似鸭传染性浆膜炎的病例中分离获得2株鸭疫里默菌(RA),通过细菌形态观察、生化试验、PCR种特异性鉴定及动物回归试验,确定2个分离株均为RA1型,分别命名为ZQ和YJ株。利用试管2倍稀释法测定了阿莫西林及阿莫西林与舒巴坦复合剂对2株RA的最小抑菌浓度(MIC)。结果表明,对阿莫西林敏感的ZQ株MIC值为0.5μg/mL,而不同比例复合剂的MIC最低值为0.007 25μg/mL,降低了100多倍。对阿莫西林耐药的YJ株的MIC值为640μg/mL,复合剂的抑菌作用明显优于阿莫西林,MIC值降低了320倍,最低值为20μg/mL。舒巴坦可以使RA对阿莫西林恢复敏感性,其MIC值明显降低,显示联合用药具有明显的的优越性。The two Rimerella anatipestifer strains were isolated from two duck farms in Guangdong province.The serotyping results indicated the serotype of R.anatipestifer in Guangdong was serotype 1,named Z Q and Y J strains respectively.The tube diulution method was used to determination of amoxicillin and sulbactam complex agent including amoxicillin and sulbactam on RA minimal inhibitory concentration（MIC）.Z Q strains was sensitive to amoxicillin,MIC was 0.5μg/mL,whereas to complex agent,MIC was 0.007 25μg/mL,which decreased 100 times as much.YJ strains was resistance to amoxicillin,MIC was 640μg/mL,whereas to complex agent,MIC was 20μg/mL,which decreased 320 times as much.Sulbactam can restore sensitivity of RA to amoxicillin,and make its MIC decrease,drug combination has obvious superiority.

关 键 词：鸭疫里默菌 药敏试验 最小抑菌浓度 联合用药 

分 类 号：S852.615[农业科学—基础兽医学] S859.796[农业科学—兽医学]