miR-19b对巨噬细胞ABCA1表达的影响  

miR-19b Regulates the Expression of Macrophage ABCA1

作　　者：吕运成[1,2] 彭超[3] 姚峰[1] 唐艳艳[1] 张熠[3] 徐菁[2] 刘政海[2] 唐朝克[1] 

机构地区：[1]南华大学医学院心血管疾病研究所 [2]南华大学临床应用解剖研究室,湖南衡阳421001 [3]南华大学附属第一医院临床研究所

出　　处：《中南医学科学杂志》2014年第4期348-350,共3页Medical Science Journal of Central South China

基　　金：湖南省自然科学基金资助项目(编号14JJ2091);湖南省教育厅资助科研项目(12C0339)

摘　　要：目的观察miR-19b对巨噬细胞ABCA1表达的影响。方法在THP-1源性巨噬细胞中转染miR-19b模拟物(mimic)和抑制物(inhibitor),荧光定量PCR检测ABCA1 mRNA,Western blot检测ABCA1蛋白水平。结果转染miR-19b mimic后,巨噬细胞ABCA1 mRNA及蛋白水平均显著下调;转染miR-19b inhibitor后ABCA1蛋白则明显上调,但ABCA1 mRNA无变化。结论 miR-19b下调巨噬细胞ABCA1的表达。Objective To investigate the impact of miR-19b on the expression of macrophage ABCA1. Methods The protein and mRNA levels of ABCA1 were detected by Western blot and quantitative real time PCR（ qRT-PCR） respectively in THP-1 macrophage transfected with miR-19b mimic and inhibitor. Results The protein and mRNA levels of ABCA1 were obviously downregulated in THP-1 macrophage transfected with miR-19b mimic. The protein level of ABCA1was evidently upregulated in THP-1 macrophage transfected with miR-19b inhibitor,while ABCA1 mRNA level had no significantly alternation. Conclusion miR-19b regulates the expression of macrophage ABCA1.

关 键 词：miR-19b ABCA1 巨噬细胞 

分 类 号：R541.4[医药卫生—心血管疾病]