中药海尔福对老年痴呆症小鼠模型干预的实验研究  

Research in Traditional Chinese Medicinal Materials Haier Fu on the Model Mice with Senile Dementia

作　　者：何胜[1] 杨海龙[1] 吴艳[1] 蓝晓惠 韦凤整 张仲将 黄春燕[1] 张树球[1] 

机构地区：[1]右江民族医学院重金属与氟砷毒物研究实验室,中国百色533000

出　　处：《国际老年医学杂志》2014年第4期145-147,162,共4页International Journal of Geriatrics

基　　金：广西自然科学基金（桂科自0991282 桂科自0499013）

摘　　要：目的：研究中药海尔福对铝致老年痴呆症（阿尔茨海默病， AD）小鼠模型大脑与血清相关的酶、生化指标及形态学的影响。方法：用氯化铝拌饲料给老鼠喂养，连续3个月，建立拟老年痴呆症模型，造模2个月后，分成3组：治疗1组、治疗2组、模型组，另设第4组为正常组，用两种不同剂型的海尔福口服液中药制剂对治疗1组、治疗2组灌胃一个月治疗。模型组和正常组作为对照组，用等体积蒸馏水代替。实验结束，取小鼠血清，处死小鼠后取大脑做成10％的脑匀浆。测定脑匀浆的乙酰胆碱转移酶（ ChAT）活性、 O·2清除率（％）、铝的含量和血清胆固醇的含量，部分大脑送病理室检查。结果：治疗1组、治疗2组、模型组和正常组依次为， ChAT （μ/g湿组织）分别为86.70±16.26、91.52±21.01、29.73±10.25、79.51±24.17，与模型组比较， P＜0.01，差异有统计学意义； O ·2清除率（％）依次为，27.12±2.68、29.71±3.81、11.31±0.16、25.56±1.90，与模型组比较， P＜0.01，差异有统计学意义；血清胆固醇（ mmol/L ）分别为3.35±0.34、2.83±0.41、4.11±0.25、3.10±0.36，与模型组比较， P ＜0.01，差异有统计学意义。脑铝含量（μg/L）分别是：52.30±2.50、98.65±8.32、236.21±13.34、54.15±2.80，与模型组比较， P＜0.05，差异有统计学意义。脑病理检查结果，正常对照：大脑海马区神经细胞、神经胶质细胞无变性、坏死，组织结构及层次未见著变。模型组：大脑皮质及海马区神经细胞、神经胶质细胞弥漫性变性、坏死、核固缩及微小软化灶形成。治疗1组、治疗2组，与模型组病变程度基本一致，但较轻。结论：从结果表明，铝中毒模型组脑乙酰胆碱转移酶活力明显低于正常组，铝含量明显高于正常组，大脑海马区神经细胞变性、坏死，治疗组明显改善。OBJECTIVE： To study the effect of Haierfu and aluminum on the brain , enzymes, serum biochemical indexes and morphology in the model mice with Alzheimer ’ s disease （ AD ） . METHODS： The mice were fed with feed mixed by Aluminium chloride which lasted three consecutive months .The model mice with AD was establisted .After two months they were divided into three groups： treatment group 1, treatment group 2, model group and normal group .Using two different formulations of Haier Fu oral liquid of Chinese traditional medicine preparation cured treating group 1 and treatment group 2 for a month.Model group and normal group ware as control group which were fed by equal volume of distilled water instead of traditional Chinese Medicine Haier Fu .After the experiment 10%brain homogenate was made with the mice serum and brain .The activity of choline acetyl transferase ChAT , ace-tyl cholinesterase, AchE, aluminum content and the content of serum cholesterol were determined and the pathological examination of part of the brain was examied .RESULTS： In treatment group 1, group 2, the model group and normal group ChAT were respectively 86.70 ±16.26, 91.52 ±21.01, 29.73 ±10.25 and 9.51 ±24.17 （μ/g wet tissue） （P〈0.01）; O·2 MPA 27.12 ±2.68, 29.71 ±3.81 , 11.31 ±0.16 and 25.56 ±1.90 （ P〈0.01 ）; Serum cholesterol 3.35 ±0.34 , 2.83 ±0.41 , 4.11 ±0.25 and 3.10 ± 0.36 （ mmol/L） （ P〈0.01 ） .All of them above compared with model group so that there were statistically significant difference . Compared with model group brain aluminum content respectively were 52.30 ±2.50 , 98.65 ±8.32 , 236.21 ±13.34 and 54.15 ± 2.80 （μg/L）.There was statistically significant difference （P〈0.05）.Pathological examination result： in the normal control, the cerebral cortex and hippocampus neural cells , glial cells degeneration and necrosis , organization structure and the level did not signifi-cantly change; in model group , the cerebral cortex and hippocampus neural cells , glial ce

关 键 词：铝 阿尔茨海默病 海尔福口服液 乙酰胆碱转移酶 

分 类 号：R135[医药卫生—劳动卫生]