石榴多酚对小鼠急性酒精性肝损伤的保护作用  

Effects and mechanisms of punicosides on acute alcoholic liver damage in mice

作　　者：魏芳[1] 许瑞杰[1] 蔡诗怡 李站站 李洁[1] 刘浩[1] 

机构地区：[1]蚌埠医学院药学系安徽省生化药物工程技术研究中心,安徽蚌埠233030

出　　处：《中国中药杂志》2014年第14期2721-2725,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81000992;81072207);国家级大学生创新创业训练计划项目(201210367018);安徽省高校省级优秀青年人才基金(2011SQRL085)

摘　　要：目的:观察石榴多酚(punicosides,Puns)对乙醇所致的急性小鼠肝损伤的保护作用,并初步探讨其作用机制。方法:将60只小鼠随机分为正常对照组、模型组、Puns低、中、高3个剂量组和水飞蓟宾组。预先给予Puns组和水飞蓟宾组灌胃给药4周后建立乙醇所致的急性肝损伤动物模型,观察Puns对肝、胸腺和脾脏指数的影响,检测小鼠血清中丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)和甘油三酯(TG)的含量,及肝匀浆中的超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽S转移酶(GSH-ST)的含量变化。HE染色观察肝脏组织学改变,免疫组化法检测肝组织中单核细胞趋化蛋白-1(MCP-1)和核转录因子-κB(NF-κB)的表达情况。结果:Puns中剂量和高剂量组能显著降低小鼠肝指数,改善肝脏脂肪变性情况,降低小鼠血清中的ALT,AST,TG水平,降低肝匀浆中的MDA含量,增加SOD,GSH-Px和GSH-ST 3种酶的活性,改善肝组织学变化,抑制肝组织中MCP-1和NF-κB的表达。结论:Puns对小鼠急性酒精性肝损伤具有保护作用。Objective： To evaluate the protective effects of punicosides on alcohol induced acute liver injury in mice and its possible mechanisms as well. Method： The 60 mice were randomly divided into normal control,model group,three dose groups of punicosides with low,medium and high,then there is silibinin group. Three dose groups of punicosides and silibinin were given in advance by gavage for 4 weeks,then the mouse model of alcoholic acute liver injury was established. The serum levels of ALT,AST and TG were determined,and the mice were killed to calculate somatic index of liver,thymus as well as spleen. MDA,SOD,GSH-Px and GSH-ST were detected in the liver homogenate. Histopathological changes of the liver were observed by HE staining. The expression of MCP-1 and NF-κB in the liver tissues were detected by immunohistochemistry. Result： Mid and high dose of punicosides reduced the liver index in mice significantly,improved liver steatosis,decreased the level of ALT,AST and TG in serum and the content of MDA in liver homogenate,furthermore the two dose groups increased the activity of SOD,GSH-Px and GSH-ST,inhibited the expression of MCP-1 and NF-κB in liver tissue. Conclusion： Punicosides can protect the acute liver damage induced by alcohol.

关 键 词：石榴多酚 酒精性肝损伤 氧化应激 MCP-1 NF-ΚB 

分 类 号：R285.5[医药卫生—中药学]