利用四引物扩增受阻突变体系PCR技术检测水稻光温敏核不育基因p/tms12-1  

Detection of Gene p/tms12-1 for Photoperiod-and Thermo-Sensitive Genic Male Sterility by Tetra-Primer Amplication Refractory Mutation System PCR in Rice

作　　者：李军[1] 李白[1] 高荣村[1] 

机构地区：[1]嘉兴市农业科学研究院,浙江嘉兴314016

出　　处：《中国水稻科学》2014年第4期442-446,共5页Chinese Journal of Rice Science

基　　金：嘉兴市科技计划资助项目(2013BY26006;2012AZ2021;2013BZ26002)

摘　　要：根据p/tms12-1基因存在的单核苷酸变异,利用四引物扩增受阻突变体系PCR(Tetra-primer ARMS-PCR)的方法设计特异引物,对11个两系不育系水稻品种(或品系)以及2个常规稻品系进行扩增。根据其PCR产物带型,可以准确鉴定光温敏核不育基因p/tms12-1的基因型,其检测结果与通过CAPS标记检测的结果完全一致。该方法成本低、简单、可靠,可用于p/tms12-1基因的鉴定和分子标记辅助育种。Specific primers were designed for tetra-primer amplification refractory mutation system PCR（ARMS-PCR）method according to the single nucleodide mutation in gene p/tms12-1.Eleven two-line sterile lines and two normal varieties were analyzed by this method.The results could clearly distinguish the genotype of p/tms12-1,which was completely consistent with the result of CAPs marker-based analysis.Therefore,as a low-cost,simple and reliable technique,this method could be widely used to the p/tms12-1 detection and molecular marker-assisted breeding in rice.

关 键 词：水稻 光温敏核不育 p/tms12-1基因 四引物扩增受阻突变体系PCR 

分 类 号：S511[农业科学—作物学]