大口黑鲈北方亚种和佛罗里达亚种mtDNA COⅠ序列的分析  

Sequence analysis of mtDNA COⅠ region in northern and Florida subspecies of largemouth bass Micropterus salmoides

作　　者：张大莉[1] 董仕[1] 白俊杰[2] 苗建发 胡莹[1] 李胜杰[2] 苗建新 张兴华[1] 马冬梅[2] 

机构地区：[1]天津师范大学生命科学学院天津市动植物抗性重点实验室,天津300387 [2]中国水产科学研究院珠江水产研究所,广州510380 [3]天津市天祥水产有限责任公司,天津301500

出　　处：《大连海洋大学学报》2014年第3期212-216,共5页Journal of Dalian Ocean University

基　　金：天津市农业科技成果转化与推广项目(201104120);科技部农业科技成果转化基金资助项目(2012GB23260562)

摘　　要：采用PCR技术对大口黑鲈Micropterus salmoides北方亚种（21尾）和佛罗里达亚种（19尾）的mtDNA COⅠ区段进行扩增,得到约1400 bp的扩增片段,经测序获得了碱基排列顺序清晰的1337 bp片段。北方亚种个体间没有变异,只检测到1种单倍型,与GenBank中大口黑鲈全序列（DQ536425）中的COⅠ区段相应部位比对,有5个碱基出现置换;佛罗里达亚种检测到5种单倍型,19个个体与本研究中的北方亚种比对,有41个碱基出现完全置换。将6种单倍型的序列提交GenBank后获得序列号为KF176376～KF176381,运用Mega 4.0软件计算两个亚种的碱基组成和碱基差异,北方亚种的单倍型多样性指数（H）、核苷酸多样性指数（Pi）和平均核苷酸差异性（k）均为0,佛罗里达亚种的H、Pi、k分别为0.784、0.003 42和4.573,两个亚种平均Kimura 2-parameter遗传距离为0.0351。研究表明,大口黑鲈佛罗里达亚种mtDNA COⅠ区段的遗传多样性高于北方亚种,该序列可作为鉴别两个亚种的DNA条形码。The PCR technology was used to amplify the mtDNA COⅠ region of 21 individuals of northern subspecies and 19 individuals of Florida subspecies of largemouth bass Micropterus salmoides,and about 1400 bp DNA fragments were obtained,in which the sequence of 1337 bp fragment were obtained by sequencing.Comparing with COⅠ region of mtDNA complete sequence of largemouth bass（ GenBank：DQ536425）,there were five base substitutions and the haplotype detected was only one within northern subspecies.There were five haplotypes within Florida subspecies,and the 41 bases substitution was detected in all individuals of Florida subspecies compare with northern subspecies.The six haplotypes has GenBank serial numbers from KF176376 to KF176381 and the base composition and base differences were calculated by Mega 4.0 software,with haplotype diversity index（ H） of 0,nucleotide diversity index（ Pi） of 0 and average nucleotide diversity（ k） of 0 in northern subspecies.Florida subspecies has H of 0.784,Pi of 0.003 42 and k of 4.573.The two subspecies have average Kimura 2-parameter genetic distance of 0.035 1.There was higher genetic diversity in mtDNA COⅠ region in Florida subspecies than in northern subspecies,indicating that the sequence of mtDNA COⅠ region can be used as a DNA barcode for identification of the two subspecies of largemouth bass.

关 键 词：大口黑鲈 COⅠ序列 遗传多样性 遗传距离 NJ系统树 

分 类 号：S965.199[农业科学—水产养殖] Q953[农业科学—水产科学]