土家药延龄草抗SH-SY5Y细胞谷氨酸损伤有效部位筛选研究  

Study on effective parts screening of Tujia medicine birthroot against damages of glusate in SH-SY5Y cells

作　　者：李志勇[1] 张嫚[1] 赵晖[2] 宋阔魁[1] 毕天[1] 周凤琴[3] 李彦文[4] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院,北京100081 [2]首都医科大学中医药学院,北京100069 [3]山东中医药大学药学院,济南250355 [4]中国中医科学院中医药信息研究所,北京100700

出　　处：《中华中医药杂志》2014年第7期2125-2129,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年基金项目(No.81001693);北京市教委青年英才项目(No.YETP1293);中央高校基本科研业务费专项(中央民族大学学术工作坊)(No.201223)~~

摘　　要：目的:筛选土家药延龄草中抗SH-SY5Y细胞谷氨酸(Glu)损伤的有效部位。方法:Bliss法测定延龄草不同部位提取物的1%致死量(LD1)和半数致死量(LD50),建立Glu损伤SH-SY5Y细胞模型,筛选延龄草中抗SH-SY5Y细胞Glu损伤的有效部位,以形态学、细胞存活率和LDH漏出率为指标评价延龄草有效部位对Glu损伤细胞的保护作用。结果:TTM1为抗SH-SY5Y细胞Glu损伤的有效部位,能显著改善Glu损伤细胞的形态,5.0、10.0、20.0μg/mL的TTM1提高损伤细胞的存活率(P<0.05,P<0.001),2.5、5.0、10.0、20.0μg/mL的TTM1可显著降低损伤细胞的LDH漏出率(P<0.05,P<0.001)。结论:延龄草TTM1对Glu损伤的SH-SY5Y细胞具有保护作用。Objective： To screen the effective parts of Tujia medicine birthroot against the damage of glusate（Glu） in SH-SY5Y cells. Methods： Bliss method was used to test the LD1 and LD50 of birthroot＇s dif erent extractives. The SH-SY5Y cells model induced by Glu was established, and effective parts against the Glu damage in SH-SY5Y cells of birthroot were screened. The protective effect of the effective part of birthroot was evaluated by the survival rate, LDH leakage rate and morphology changes. Results： TTM1 was the effective part of birthroot, which could protect the SH-SY5Y cells against Glu injury. The survival rate of damaged cells could be improved by 5.0, 10.0 and 20.0μg/mL TTM1（P〈0.05, P〈0.001）. The LDH leakage rate of damaged cells could be reduced signii cantly by 2.5, 5.0, 10.0 and 20.0μg/mL TTM1（P〈0.05, P〈0.001）. Conclusion： TTM1 of birthroot could protect the SH-SY5Y cells damaged by Glu.

关 键 词：延龄草 谷氨酸损伤 有效部位筛选 SH-SY5Y细胞 

分 类 号：R29[医药卫生—民族医学]