复方菖蒲益智汤对脑缺血再灌注损伤小鼠IL-1β及TGF-β1表达水平的影响  

Effect of Fufang Changpu Yizhi Decoction on the level of IL-1β and TGF-β1 in mice of cerebral ischemia-reperfusion injury

作　　者：田军彪[1] 臧春柳 崔媛[3] 徐丽娟[1] 刘学飞[1] 赵见文[4] 

机构地区：[1]河北省中医院,石家庄050011 [2]河北省唐县中医院,保定072350 [3]石家庄市中医院,石家庄050051 [4]河北省沧州中西医结合医院,沧州061001

出　　处：《中华中医药杂志》2014年第7期2309-2311,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药管理局中医药行业科研专项(No.201007002);河北省科技支撑计划项目(No.12276104D-79);河北省中医药管理局科研计划项目(No.2012031)~~

摘　　要：目的:观察复方菖蒲益智汤对脑缺血再灌注损伤小鼠白介素-1β(IL-1β)、转化生长因子-β1(TGF-β1)表达水平的影响,探讨复方菖蒲益智汤防治脑缺血再灌注损伤的可能机制。方法:采用阻断双侧颈总动脉法制备脑缺血再灌注模型。放射免法检测各组小鼠血清中IL-1β的表达水平,免疫组织化学法检测各组小鼠脑组织TGF-β1的表达水平。结果:与假手术组相比,模型组小鼠IL-1β、TGF-β1的表达水平明显升高(P<0.05);与模型组相比,各用药组小鼠血清中炎症因子IL-1β表达水平明显降低(P<0.05),脑组织TGF-β1的表达水平明显升高(P<0.05),而复方菖蒲益智汤高剂量组尤为明显(P<0.05)。结论:复方菖蒲益智汤可能通过调节炎症因子的释放,抑制炎症反应,降低脑缺血再灌注损伤的程度。Objective： To observe the inl uence of Fufang Changpu Yizhi Decoction on the level of IL-1β and TGF-β1 in mice of cerebral ischemia-reperfusion injury（CIRI） and to explore the possible mechanisms on prevention and treatment for cerebral ischemia-reperfusion injury with Fufang Changpu Yizhi Decoction. Methods： Animal model of cerebral ischemiareperfusion injury was established by using the method of blocking bilateral common carotid artery. Radioimmunoassay was used to test the expression level of IL-1β in serum of mice of all groups. Immunohistochemisty was used to test the expression level of TGF-β1 in brain tissue of mice. Results： Compared with the sham operation group, the level of IL-1β and TGF-β1 in the model group increased signii cantly（P〈0.05）. Compared with model group, the level of IL-1β of treatment groups decreased signii cantly（P〈0.05）, but the level of TGF-β1 of each dose group increased signii cantly in brain tissue（P〈0.05）, especially the high dose group of Fufang Changpu Yizhi Decoction（P〈0.05）. Conclusion： Fufang Changpu Yizhi Decoction could reduce the degree of CIRI via adjusting the release of inflammatory factors to reduce inflammatory reaction so as to lower the intensity of cerebral ischemia-reperfusion injury.

关 键 词：脑缺血再灌注损伤 复方菖蒲益智汤 化浊解毒活血通络法 炎症反应 转化生长因子-Β1 白介素-1Β 

分 类 号：R285.5[医药卫生—中药学]