熊胆粉对人脐静脉内皮细胞增殖与迁移作用的实验研究  

Experimental study on the effect of bear gall powder(BBP) on proliferation and migration of human umbilical vein endothelial cells

作　　者：褚剑锋[1,2] 沈阿灵[2] 刘丽雅[2] 李颖[2] 赵锦燕[2] 许剑峰 彭军[2] 洪振丰[2] 

机构地区：[1]福建归真堂药业股份有限公司博士后科研工作站,泉州350122 [2]福建中医药大学中西医结合研究院,福州350122

出　　处：《中华中医药杂志》2014年第7期2349-2351,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：福建省科技重大专项(No.2010YZ0001-2);福建省教育厅A类科研项目(No.JA12178)~~

摘　　要：目的:确定熊胆粉(BBP)对人脐静脉内皮细胞(HUVEC)增殖及迁移能力的影响。方法:MTT法检测BBP干预下HUVEC增殖活力的影响;划痕实验分析BBP对HUVEC迁移能力的影响。结果:BBP干预24h后,HUVEC的增殖活力受到抑制,尤其到72h时,各药物浓度下HUVEC增殖活力受到显著抑制(P<0.05,P<0.01),以药物浓度0.3、0.6、0.9mg/mL分别干预24、48、72h后,表现出时间与药物浓度依赖的特征,HUVEC的迁移能力也受到明显抑制。结论:BBP对HUVEC的增殖与迁移能力有显著的抑制作用,表现出潜在的抑制血管生成的活性。Objective： To determine the effect of bear gall powder（BBP） on proliferation and migration ability of human umbilical vein endothelial cells（HUVEC）. Methods： To test the HUVEC proliferation activity by using MTT. The scratch test was used to analyze HUVEC migration ability by BBP intervention. Results： After 24h BBP intervention, HUVEC proliferation activity was inhibited signii cantly, especially after 72 hours, the HUVEC proliferation activity was inhibited（P〈0.05, P〈0.01）. After 24, 48, 72 hours intervention and concentration of medicine in 0.3, 0.6, 0.9mg/mL respectively, it showed the characteristics of time and drug concentration. The HUVEC migration ability was also signii cantly inhibited. Conclusion： BBP has a signii cantly inhibition on proliferation and migration ability of HUVEC and shows potential anti-angiogenesis activity.

关 键 词：熊胆粉 人脐静脉内皮细胞 细胞活力 迁移能力 

分 类 号：R285[医药卫生—中药学]