Study on Major Factors Influencing the Proliferation and Growth of Aseptic Buds of Vietnam Mesona blumes  

作　　者：Pengxiao WEI Muxiu TAN Xiufen CEN Liulan QIN Jieyun LIU Fangfang ZHOU 

机构地区：[1]Agricultural College,Guangxi University

出　　处：《Agricultural Biotechnology》2014年第3期7-10,共4页农业生物技术（英文版）

基　　金：Supported by Scientific Research and Technological Development Project of Wuzhou City,Guangxi Zhuang Autonomous Region(200901024)

摘　　要：[Objective]This study aimed to investigate the major factors influencing the proliferation and growth of aseptic buds of Vietnam Mesona blumes,thus providing technical references for establishing rapid propagation system in vitro of Vietnam M.blumes.[Method]The effects of medium components（different basic medium,sucrose concentrations and cytokinins）and explant materials（different explants）on the proliferation of aseptic buds of Vietnam M.blumes were analyzed.[Result]Among MS,Miller and modified White basic medium,MS medium exhibited the best effect on proliferation of Vietnam M.blumes buds with the bud proliferation multiple of 8.53.Among different sucrose concentrations,20.0 g/L sucrose gave the highest bud proliferation multiple of 8.34,while 25.0 g/L sucrose led to the best growth status with the bud proliferation multiple of 7.05.The effect of CPPU on bud proliferation was higher than that of 6-BA and KT,with the bud proliferation multiple of 9.80.Among different explants,top stem exhibited the best effect on bud proliferation with the bud proliferation multiple of 9.35,resulting in robust seedlings.[Conclusion]The most suitable subculture medium for the proliferation and growth of aseptic buds of Vietnam M.blumes is MS＋20.0-25.0 g/L sucrose＋0.5 mg/L CPPU and the most suitable explant is top stem.[Objective]This study aimed to investigate the major factors influencing the proliferation and growth of aseptic buds of Vietnam Mesona blumes,thus providing technical references for establishing rapid propagation system in vitro of Vietnam M.blumes.[Method]The effects of medium components（different basic medium,sucrose concentrations and cytokinins）and explant materials（different explants）on the proliferation of aseptic buds of Vietnam M.blumes were analyzed.[Result]Among MS,Miller and modified White basic medium,MS medium exhibited the best effect on proliferation of Vietnam M.blumes buds with the bud proliferation multiple of 8.53.Among different sucrose concentrations,20.0 g/L sucrose gave the highest bud proliferation multiple of 8.34,while 25.0 g/L sucrose led to the best growth status with the bud proliferation multiple of 7.05.The effect of CPPU on bud proliferation was higher than that of 6-BA and KT,with the bud proliferation multiple of 9.80.Among different explants,top stem exhibited the best effect on bud proliferation with the bud proliferation multiple of 9.35,resulting in robust seedlings.[Conclusion]The most suitable subculture medium for the proliferation and growth of aseptic buds of Vietnam M.blumes is MS＋20.0-25.0 g/L sucrose＋0.5 mg/L CPPU and the most suitable explant is top stem.

关 键 词：Vietnam Mesona blumes Aseptic seedling Bud proliferation Influencing factors 

分 类 号：S567.219[农业科学—中草药栽培]