A Comparison of the Effects of Pentoxifylline on Quality of Fresh and Frozen-thawed Bull Spermatozoa  

作　　者：Jian ZHANG Huamin GUO Jie SU Lixia ZHAO Yunxia LI Wei SUN Hongmei HAN Shuxiang HU Gaoping ZHAO Yao LI Yanfeng DAI Xihe LI 

机构地区：[1]Research Center for Animal Genetic Resources of Mongolia Plateau,Inner Mongolia University [2]Inner Mongolia Saikexing Reproductive Biotechnology Co. Ltd.

出　　处：《Agricultural Biotechnology》2014年第3期20-24,29,共6页农业生物技术（英文版）

基　　金：Supported by NMKJ Project of Production and Industrial Application of Sexed Semen in Domestic Animals(No.20111701)

摘　　要：[Object]This study aimed to examine the effects of 3,7-dimethyl-1-（5-oxo-hexyl）-xanthine（pentoxifylline,PF）on the motility,mitochondrial activity,acrosome integrity and fertilization rate of spermatozoa in both fresh and frozen-thawed bull semen.[Method]Fresh and frozen-thawed bull spermatozoa were exposed to 5 mmol/L PF with untreated samples as controls.[Result]Fresh spermatozoa showed reduced（P〈0.05）motility after 2 h incubation with PF whereas,surprisingly,frozen-thawed samples exhibited increased sperm motility（P〈0.05）after 2 h incubation with PF and they also showed enhanced longevity compared to controls.Mitochondrial activity in both fresh and frozen-thawed bull spermatozoa increased（P〈0.05）during 4 h incubation with PF whereas acrosome integrity remained unchanged in both types of semen.However,treatment with 5 mmol/L PF did not influence the in vitro fertilization efficiency of fresh spermatozoa but improved significantly（P〈0.05）that of frozen-thawed spermatozoa.[Conclusion]These results indicate that PF can improve sperm quality of frozenthawed bull semen,and may improve pregnancy rates in bovine artificial insemination programmes employing frozen semen.[Object]This study aimed to examine the effects of 3,7-dimethyl-1-（5-oxo-hexyl）-xanthine（pentoxifylline,PF）on the motility,mitochondrial activity,acrosome integrity and fertilization rate of spermatozoa in both fresh and frozen-thawed bull semen.[Method]Fresh and frozen-thawed bull spermatozoa were exposed to 5 mmol/L PF with untreated samples as controls.[Result]Fresh spermatozoa showed reduced（P〈0.05）motility after 2 h incubation with PF whereas,surprisingly,frozen-thawed samples exhibited increased sperm motility（P〈0.05）after 2 h incubation with PF and they also showed enhanced longevity compared to controls.Mitochondrial activity in both fresh and frozen-thawed bull spermatozoa increased（P〈0.05）during 4 h incubation with PF whereas acrosome integrity remained unchanged in both types of semen.However,treatment with 5 mmol/L PF did not influence the in vitro fertilization efficiency of fresh spermatozoa but improved significantly（P〈0.05）that of frozen-thawed spermatozoa.[Conclusion]These results indicate that PF can improve sperm quality of frozenthawed bull semen,and may improve pregnancy rates in bovine artificial insemination programmes employing frozen semen.

关 键 词：Bull spermatozoa MOTILITY Mitochondrial activity Acrosome integrity FERTILIZATION 

分 类 号：S823[农业科学—畜牧学]