Cloning and Sequence Analysis of Mutant OmpF from Antibiotic Resistant Escherichia coli  

作　　者：Xin NIE Li ZHANG Tingting LIU Yang ZHAO Zaixin LI Zhiping ZHAO 

机构地区：[1]School of Chemistry and Pharmaceutical Engineering,Sichuan University of Science & Engineering

出　　处：《Agricultural Biotechnology》2014年第3期50-52,共3页农业生物技术（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.31100089);Scientific Research Foundation of the Education Department of Sichuan Province(No.11ZB102);the Talent Project of Sichuan University of Science&Engineering(No.2011RC12)

摘　　要：[Objective]This study aimed to investigate the mutations of OmpF from an isolated antibiotic resistant Escherichia coli strain.[Methods]The mutant OmpF（mOmpF）from antibiotic resistant E.coli was amplified by PCR with Pfu DNA polymerase and ligated into the expression vector pET28a.Subsequently,the expression vector pET28-mOmpF was sequenced and analyzed by DNAMAN software and Swiss-Model online.[Result]Sequence analysis revealed that the open reading fragment of mOmpF was 903 bp long,which was mutated dramatically compared to that of the 1 020 bp long model OmpF.The DNA sequence shared only54.5%homology with OmpF.mOmpF was 44.6%identical to that of OmpF.Protein structure predication and analysis through Swiss-Model online suggested that the structure of mOmpF changed dramatically compared to OmpF.[Conclusion]The present study provided basis for further analyzing the relationships between the structure and functions of mOmpF from antibiotic resistant E.coli.[Objective]This study aimed to investigate the mutations of OmpF from an isolated antibiotic resistant Escherichia coli strain.[Methods]The mutant OmpF（mOmpF）from antibiotic resistant E.coli was amplified by PCR with Pfu DNA polymerase and ligated into the expression vector pET28a.Subsequently,the expression vector pET28-mOmpF was sequenced and analyzed by DNAMAN software and Swiss-Model online.[Result]Sequence analysis revealed that the open reading fragment of mOmpF was 903 bp long,which was mutated dramatically compared to that of the 1 020 bp long model OmpF.The DNA sequence shared only54.5%homology with OmpF.mOmpF was 44.6%identical to that of OmpF.Protein structure predication and analysis through Swiss-Model online suggested that the structure of mOmpF changed dramatically compared to OmpF.[Conclusion]The present study provided basis for further analyzing the relationships between the structure and functions of mOmpF from antibiotic resistant E.coli.

关 键 词：mOmpF Outer membrane protein OMPF Antibiotic resistance 

分 类 号：Q933[生物学—微生物学]