芦根多糖对高脂诱导大鼠肾损伤的保护作用  

Protective effect of Rhizoma Phragmitis Polysaccharide on kidney in hyperlipidemia-induced rats

作　　者：徐行仙[1] 

机构地区：[1]浙江省江山市人民医院药剂科,浙江江山324100

出　　处：《中国医药导报》2014年第19期24-27,共4页China Medical Herald

摘　　要：目的探讨芦根多糖对高脂诱导大鼠肾损伤的保护作用。方法制备高脂诱导大鼠肾损伤模型,将高脂大鼠分成3组,每组各10只,分别为芦根多糖高(300 mg/kg)、低剂量组(150 mg/kg)和对照组,其中高剂量组每日灌胃给予芦根多糖300 mg/kg,低剂量组每日灌胃给予芦根多糖150 mg/kg,对照组每日给予等量纯净水,连续灌胃13周,测定24 h尿蛋白定量、血清肌酐(Cr)、尿素氮(BUN)、总胆固醇(TC)、三酰甘油(TG)、肾脏丙二醛(MDA)、超氧化物歧化酶(SOD)、低密度脂蛋白(LDL-C)、氧化低密度脂蛋白(Ox-LDL)及肾小球内径。结果与对照组比较,芦根多糖高剂量组2 h尿蛋白定量、血清BUN、血清Cr、TC、TG、肾组织MDA、LDL-C、Ox-LDL等明显降低(P<0.05或P<0.01),肾组织SOD明显升高(P<0.01),显微结构肾小球内径明显减小(P<0.05);而低剂量组虽有一定的改善,但差异无统计学意义(P>0.05)。结论芦根多糖具有降脂抗氧化作用,能减少脂质过氧化产物,减轻对肾脏的损伤,同时具有减轻尿蛋白排泄及减小肾小球内径的作用,因此其对高脂造成大鼠肾损害具有一定的保护作用。Objective To investigate the protective effect of Phragmitis Polysaccharide against fat-induced renal injury in rats. Methods The model for Rhizoma Phragmitis Polysaccharide on kidney in hyperlipidemia-induced rats was made, and high-fat rats were divided into three groups, which were high-dose group（300 mg/kg）, low-dose group（150mg/kg） and the control group with 10 rats in each group. Rats were given Rhizoma Phragmitis Polysaccharide for 300,150 mg/kg and amount of pure water by gavage for 13 weeks, respectively, then 24-hour urinary protein excretion,serum creatinine（Cr）, blood urea nitrogen（BUN）, total cholesterol（TC）, triglyceride（TG）, kidney malondialdehyde（MDA）, superoxide dismutase enzyme（SOD）, low-density lipoprotein（LDL-C）, oxidized low-density lipoprotein（OxLDL） and glomerular inside diameter were measured. Results Compared with the control group, high-dose group with24-hour urinary protein excretion and serum BUN, serum Cr, TC and TG renal tissue MDA, LDL-C and Ox-LDL were significantly lower（P〈0.05 or P〈0.01）, renal tissue SOD increased significantly（P〈0.01）, the inside diameter of the microstructure of the glomerular reduced significantly（P〈0.05）; while low doses had some improvement with above index, but withnot statistically significant（P〉0.05）. Conclusion Phragmites polysaccharide can reduce lipid peroxidation, reduce urinary protein excretion and glomerular diameter, so it has a protective effect on fat rat kidney damage.

关 键 词：芦根多糖 肾脏 抗氧化 

分 类 号：R285.5[医药卫生—中药学]