细胞因子信号传导抑制蛋白1调控C17.2神经干细胞向神经元分化的研究  

Suppressor of cytokine signaling 1 regulates the neuronal differentiation of C17. 2 neuronal stem cells

作　　者：崔猛[1] 戴斌[2] 冯世庆[3] 辛景义[1] 

机构地区：[1]天津医院创伤骨科,300211 [2]天津医科大学总医院内分泌科 [3]天津医科大学总医院骨科

出　　处：《中华实验外科杂志》2014年第7期1395-1398,共4页Chinese Journal of Experimental Surgery

基　　金：天津市卫生局科技基金资助项目（2013KR15）

摘　　要：目的 观察细胞因子信号传导抑制蛋白I（SOCS1）对C17.2神经干细胞（C17.2NSCs）分化的调控作用。方法 用含目的基因SOCS1的慢病毒体外感染C17.2NSCs,实验分3组：SOCS1组、空载体组、磷酸盐缓冲液（PBS）组。采用光镜观察3组细胞的形态变化,采用细胞免疫荧光染色、Western blot、逆转录-聚合酶链反应（RT-PCR）法观察3组细胞中巢蛋白（Nestin）、微管相关蛋白Ⅱ（MAP2）、β-微管相关蛋白Ⅲ（β-tubulinⅢ）、人髓鞘碱性蛋白（MBP）、胶质纤维酸性蛋白（GFAP）的表达。结果 SOCS1组中Nestin表达较其他两组减少,而MAP2表达较其他两组增加（Nestin：0.234±0.008、0.925±0.040、1.000±0.039,MAP2：2.598±0.067、0.890±0.038、1.000±0.032,P〈0.01）,SOCS1组中部分C17.2NSCs的形态向神经元演变且该部分细胞均表达β-tubulinⅢ,SOCS1组中β-tubulinⅢ阳性染色细胞数所占比例高于其他两组（SOCS1组：10.05%,空载体组：0.71%,PBS组：0.65%,P〈0.01）,SOCS1组中β-tubulinⅢ蛋白表达量较其他两组增加（β-tubulinⅢI：3.028±0.121、0.978±0.254、1.000±0.042,P〈0.01）。结论 SOCS1能够调控C17.2NSCs向神经元分化。Objective To investigate the role of suppressor of cytokine signaling 1 （SOCS1） in regulating the differentiation of C17. 2 neuronal stem cells （NSCs）. Methods C17. 2 NSCs were transfect- ed by Lentiviral vector which included objective gene-SOCS1. The experiment was individed into LV-SOCSI group, LV group and PBS group. The morphological change of C17. 2NSCs after transfeetion was observed by light microscopy. The expression of Nestin, microtubule-associated proteins 2 （MAP2）, tubulinⅢ, myelin basic protein （MBP） and glial fibrillary acidic protein （GFAP） in C17. 2 NSCs was detected by immunoeytochemistry, Western blotting and reverse transcription-polymerase chain reaction （RT-PCR）. The protein expression of β-tubulinlU in C17.2 NSCs was examined by immunocytochemistry and Western blotting. Results An decreased expression of Nestin and an increased expression of MAP2 in LV-SOCS1 group was found as compared with LV group and PBS group after transfection （P 〈 0.01 ）. Some C17.2 NSCs in LV-SOCS1 group underwent prominent neuronal morphological changes after transfection and almost all the cells with morphological changes were β-tubulin Ⅲ-positive, and the other two groups had less cells with morphological changes and β-tubulin Ⅲ-positive （LV-SOCS1 group： 10. 05%, LV group： 0. 71%, PBS group： 0. 65% ） （P 〈 0. 01 ）. The 13-tubulin Ill expression in LV-SOCS1 group was significantly higher than in other two groups （P 〈 0. 01 ）. Conclusion SOCS1 may play a role in promoting the neuronal differentiation of C17. 2 NSCs.

关 键 词：细胞因子信号传导抑制蛋白1 神经干细胞 神经元 分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]