Janus激酶2/信号转导与转录激活子3信号通路在胰蛋白酶原激活肽诱导大鼠胰腺腺泡细胞释放高迁移率族蛋白B1中的作用  

Effect of Janus activated kinase 2/signal transducer and activators of transcription 3 signaling pathway on the release of high mobility group box 1 in trypsinogen activation peptide-induced rat pancreatic acinar cells

作　　者：任浩源[2] 兑丹华[1] 刘尧[1] 王国良[1] 江小静[1] 白亮[1] 

机构地区：[1]遵义医学院附属医院肝胆胰外科,贵州563000 [2]德阳市人民医院普二科,618000

出　　处：《中华实验外科杂志》2014年第7期1511-1513,共3页Chinese Journal of Experimental Surgery

基　　金：贵州省科学技术基金资助项目（黔科合J字[2008]2200号）

摘　　要：目的 观察Janus激酶2/信号转导与转录激活子3（JAK2/STAT3）信号通路在胰蛋白酶原激活肽（TAP）诱导大鼠胰腺腺泡细胞释放高迁移率族蛋白B1（HMGB1）中的作用。方法 提取大鼠胰腺腺泡细胞,随机分为正常对照组、TAP促进组（终质量浓度为3 nmol/L）、JAK2抑制剂AG490预处理组（25μ.mol/L）、STAT3抑制剂雷帕霉素预处理组（40μg/L）,采用聚合酶链反应（PCR）及Western blot法检测3-24 h各组HMGB1 mRNA及蛋白表达水平。结果 与正常对照组HMGB1 mRNA表达水平（1.01±0.04）比较,TAP组6-24 h HMGB1 mRNA表达水平显著增高（2.87±0.08、32.85±1.48、38.43±1.60,P〈0.01）,与TAP组比较,AG490预处理组12-24 h HMGB1 mRNA表达均显著抑制（23.40±1.38、19.44±0.89,P〈0.01）,与TAP组比较,雷帕霉素预处理组12-24 h HMGB1 mRNA表达均显著抑制（21.14±1.32、17.76±0.57,P〈0.01）。与正常对照组HMGB1蛋白表达水平（0.39±0.02）比较,TAP组3-24 h HMGB1蛋白表达显著增高（0.46±0.02、0.67±0.03、0.72±0.02、0.90±0.03,P〈0.01）,与TAP组比较,AG490预处理组12-24 h HMGB1蛋白表达均显著抑制（0.60±0.02、0.54±0.01,P〈0.01）,与TAP组比较,雷帕霉素预处理组12-24 h HMGB1蛋白表达均显著抑制（0.59±0.02、0.58±0.01,P〈0.01）。结论 TAP可诱导大鼠胰腺腺泡细胞HMGB1的表达和释放,其机制可能与JAK2/STAT3信号通路有关。Objective To research the effect of Janus activated kinase 2/signal transducer and ac- tivators of transcription 3 （ JAK2/STAT3 ） signaling pathway on the release of high mobility group box 1 （ HMGB1 ） in trypsinogen activation peptide （TAP） -induced rat pancreatic acinar cells. Methods The rat pancreatic acinar cells were isolated, and randomly divided into normal control group, TAP promotion group （3 μmol/L） , AG490 treatment group （25 μmol/L） and RPM treatment group （40 μg/L）. Within 3-24 h the HMGB1 mRNA and protein expression levels were detected by using PCR and Western blotting. Results In comparison with the normal control group （ 1.01 ± 0.04）, HMGB1 mRNA expression was significantly increased at 6, 12 and 24 h in TAP promotion group （2. 87 ±0. 08, 32. 85 ± 1.48 and 38.43 ± 1.60, P 〈0. 01 for all）, that was dramatically inhibited at 12 and 24 h in AG490 treatment group as com pared with TAP promotion group （23.40 ± 1.38 and 19.44 ±0. 89, P 〈0. O1 for all）, and that was drmatically inhibited at 12 and 24 h in RPM treatment group as compared with TAP promotion group （21.14 ± 1.32 and 17.76 ± 0.57, P〈 0.01 for all）. In comparison with the normal control group （0. 39 ±0. 02）, the HMGB1 expression was significantly increased at 3, 6, 12 h and 24 h in TAP promotion group （0. 46 ± 0.02, 0. 67 ± 0. 03, 0. 72 ± 0. 02 and 0. 90 ± 0. 03, P 〈 0. 01 for all）, that was dramat-ically inhibited at 12 and 24 h in AG490 treatment group as compared with TAP promotion group （0. 60 ± 0. 02 and 0. 54 ±0. 01, P 〈0. 01 for all）, and that was dramatically inhibited at 12 and 24 h in RPM treat ment group as compared with TAP promotion group （0. 59 ± 0.02 and 0. 58 ± 0.01, P 〈 0.01 for all）. Conclusion TAP induces rat pancreatic acinar cells to release HMGB1, which is related to intracellular JAK2/STAT3 siznalinz oathwav.

关 键 词：胰腺腺泡细胞 胰蛋白酶原激活肽 高迁移率族蛋白B1 JANUS激酶2 雷帕霉素 

分 类 号：R3416[医药卫生—基础医学]