胶原基复合水凝胶的制备与表征(Ⅱ)——结构性能分析与生物学评价  

Preparation and Characterization of Polyvinyl Alcohol-Collagen Hydrogels(Ⅱ):Structure performance analysis and biological evaluation

作　　者：胡杨[1,2] 林海[3] 但卫华[1,2] 刘兰[1,2] 但年华[1,2] 

机构地区：[1]四川大学制革清洁技术国家工程实验室,四川成都610065 [2]四川大学生物医学工程技术研究中心,四川成都610065 [3]四川大学国家生物材料工程技术研究中心,四川成都610065

出　　处：《中国皮革》2014年第13期9-13,共5页China Leather

基　　金：国家自然科学基金项目(No:21276164);国家科技支撑计划项目(No:2011BAC06B11)

摘　　要：通过物理交联和化学交联并用的方式制备胶原基复合水凝胶。通过红外光谱（FT—IR）、差示量热扫描（DSC）、X射线衍射分析（XRD）、扫描电子显微镜（SEM）观察等手段，对胶原基复合水凝胶的基团组成、结晶度、结构及形貌等情况进行分析。FT-IR结果表明：水凝胶中的基团组成符合原料组成的特点，能够定性判断聚乙烯醇与胶原形成共价交联。DSC法计算的水凝胶中结晶度为27．5％左右。XRD分析中出现了聚乙烯醇的特征吸收峰，验证其晶体结构的存在。SEM结果显示胶原基复合水凝胶具有多孔结构。同时，水凝胶的溶胀特性、透水汽性良好。细胞毒性试验表明，胶原的存在能够促进成纤维细胞的生长，细胞毒性等级为0—1级，符合医用创面敷料的基本要求。The polyvinyl Mcohol - collagen hy＆ogels was prepared by the method of physical crosslinking and chemical crosslinking. The composition groups, crystallinity, structure and surface morphology of compound hydrogels were charactered by FT - IR, DSC, XRD and SEM et M. The FT-IR results indicate that the composition groups of compound hydrogels are in accordance with the composition of raw materials, and it forms covalent cross - linking between PVA and collagen. DSC resuhs show that the crystallinity of compound hydrogels is about 27.5 %. XRD results indicate that the appearance of characteristic absorption peak of PVA confirms the existence of crystal structure. SEM results show that the compound hydrogels have a porous structure, good swelling properties and water vapor permeability. The cytotoxicity experiment reveals that the presence of collagen can promote the growth of fibrocyte. And the cytotoxicity grade is 0 - 1 ,which meet the standards of biological wound dressing.

关 键 词：胶原 聚乙烯醇 水凝胶 生物学性能 

分 类 号：TQ427.26[化学工程]