胃饥饿素对大鼠急性重症胰腺炎AMY、IL-6和TNF-α水平及病理学的影响  

Effect of ghrelin on serum levels of amylase, interleukin-6 and tumor necrosis factor-α in rats with severe acute pancreatitis

作　　者：哈红萍[1] 李建华[2] 

机构地区：[1]青海大学医学院解剖教研室,青海省西宁市810000 [2]青海大学医学院基础医学部,青海省西宁市810000

出　　处：《世界华人消化杂志》2014年第16期2285-2289,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨Ghrelin对急性重症胰腺炎(severe acute pancreatitis,SAP)血清淀粉酶(amylase,AMY)、白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平及病理学的影响效果.方法:按照随机数字表法将60只SPF级Wistar大鼠均分为A、B、C、D 4组,A组大鼠不使用牛磺胆酸钠诱发SAP,B、C、D三组大鼠均使用牛磺胆酸钠诱发SAP,C组术后使用Ghrelin治疗,D组术前使用[D-Lys3]GHRP-6,术后使用Ghrelin治疗,比较4组大鼠血清AMY、IL-6、TNF-α水平、胰腺含水量,并切片比较光镜下胰腺组织病理学图像及光镜下胰腺组织病理评分.结果:C组大鼠血清AMY、IL-6和TNF-α水平均明显低于B组(3806.02 U/L±536.27 U/L vs4794.86 U/L±477.98 U/L、167.22 pg/mL±11.75 pg/mL vs 204.96 pg/mL±17.44 pg/mL、2.62 pg/mL±0.72 pg/mL vs 3.25 pg/mL±0.92pg/mL),差异具有统计学意义(P<0.05);D组大鼠血清AMY、IL-6和TNF-α水平均明显高于C组(4451.16 U/L±238.86 U/L vs 3806.02 U/L±536.27 U/L、197.73 pg/mL±17.38 pg/mL vs 167.22 pg/mL±11.75 pg/mL、3.17 pg/mL±0.69 pg/mL vs 2.62 pg/mL±0.72 pg/mL),差异具有统计学意义(P<0.05);C组和D组大鼠胰腺含水量均明显低于B组(28.65%±0.77%vs35.41%±0.78%、29.30%±0.40%vs 35.41%±0.78%),差异具有显著统计学意义(P<0.01);C组大鼠水肿、炎症、出血及坏死评分均明显低于B组(2.06分±0.41分vs 2.82分±0.55分、2.52分±0.63分vs 3.18分±0.47分、1.93分±0.64分vs 2.79分±0.58分、2.22分±0.74分vs 2.84分±0.63分),差异具有显著统计学意义(P<0.01);D组大鼠水肿、炎症、出血及坏死评分均明显高于C组(2.66分±0.32分vs2.06分±0.41分、3.09分±0.78分vs 2.52分±0.63分、2.42分±0.39分vs 1.93分±0.64分、2.63分±0.26分vs 2.22分±0.74分),差异具有统计学意义(P<0.05).结论:Ghrelin治疗能够有效改善大鼠AMY、IL-6和TNF-α水平,改善胰腺病理损害,对SAP有良好的治疗效果.AIM： To explore the effect of ghrelin on serum levels of amylase（AMY）, interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） in rats with severe acute pancreatitis.METHODS： Sixty Wistar rats were randomly divided into four groups： A, B, C and D. Groups B, C and D were treated with sodium taurocholate hydrate to induce SAP, while group A did not. Before induction of SAP, group D was treated with [D-Lys3]GHRP-6. After induction of SAP, groups C and D were treated with ghrelin. Serum levels of AMY, IL-6, and TNF-α, the moisture content of the pancreas, and the pathological changes in the pancreatic tissue were determined and compared between different groups.RESULTS： Serum levels of AMY, IL-6 and THF-α in group C were significantly lower than those in group B（3806.02 U/L ± 536.27 U/L vs 4794.86 U/L ± 477.98 U/L, 167.22 pg/mL ± 11.75 pg/mL vs 204.96 pg/mL ± 17.44 pg/mL, 2.62 pg/mL ± 0.72 pg/mL vs 3.25 pg/mL ± 0.92 pg/mL; P ＆lt; 0.05 for all）. Serum levels of AMY, IL-6 and THF-α in group D were significantly higher than those in group C（4451.16 U/L ± 238.86 U/L vs 3806.02 U/L ± 536.27 U/L, 197.73 pg/mL ± 17.38 pg/mL vs 167.22 pg/mL ± 11.75 pg/mL, 3.17 pg/mL ± 0.69 pg/mL vs 2.62 pg/mL ± 0.72 pg/mL; P ＆lt; 0.05 for all）. The moisture contents of the pancreas in groups C and D were significantly lower than that in group B（28.65% ± 0.77% vs 35.41% ± 0.78%, 29.30% ± 0.40% vs 35.41% ± 0.78%; P ＆lt; 0.01 for both）. The edema, inflammation, hemorrhage and necrosis scores in group C were significantly lower than those in group B（2.06 ± 0.41 vs 2.82 ± 0.55, 2.52 ± 0.63 vs 3.18 ± 0.47, 1.93 ± 0.64 vs 2.79 ± 0.58, 2.22 ± 0.74 vs 2.84 ± 0.63; P ＆lt; 0.01 for all）, while edema, inflammation, hemorrhage and necrosis scores in group D were significantly higher than those in group C（2.66 ± 0.32 vs 2.06 ± 0.41, 3.09 ± 0.78 vs 2.52 ± 0.63, 2.42 ± 0.39 vs 1.93 ± 0.64, 2.63 ± 0.26 vs 2.22 ± 0.74, P ＆lt; 0.05 for all）.CONCLUSION： Ghrelin can improve s

关 键 词：GHRELIN 急性重症胰腺炎 影响效果 

分 类 号：R657.51[医药卫生—外科学]