类风湿因子免疫吸附血液灌流材料的制备及其性能评价  

Study on Preparation and Properties of Immune Adsorbent for Rheumatoid Factorremoval in Hemoperfusion

作　　者：毛金春[1] 王翔[1] 张荣华 靳欣欣[1] 唐福州[1] 熊延连[1] 蒋毅 

机构地区：[1]重庆大学生物工程学院生物流变科学与技术教育部重点实验室,重庆400044 [2]第三军医大学第一附属医院中西医结合科风湿病中心,重庆400038

出　　处：《中国生物医学工程学报》2014年第3期335-342,共8页Chinese Journal of Biomedical Engineering

基　　金：重庆市科委科技计划攻关项目(cstc2011ac5193);重庆市科委基地建设费(cstc2011pt-gc0030)

摘　　要：制备一种新型的类风湿因子(RF)免疫吸附剂并研究其性能。通过在氯甲基聚苯乙烯-二乙烯树脂(氯球)的表面接枝苯丙氨酸(PHE),制备出可供临床应用的类风湿因子血液灌流吸附剂(PS-PHE);体外动态灌流实验测定吸附剂的吸附率;体外动态洗脱实验测定RF的脱落量及脱落率;通过体外灌流模拟实验,检测灌流对血液成分的影响来评价吸附剂的选择性;体外凝血酶原时间(PTs)及凝血酶时间(TTs)检测实验验证材料的血液相容性。免疫吸附剂对类风湿因子IgA RF、IgG RF、IgM RF的吸附率分别可以达到45.21%±1.80%、56.02%±1.36%、52.40%±2.01%(n=5),洗脱后脱落率分别为22.10%±1.65%、19.23%±1.06%、21.31%±1.35%(n=5)。全血灌流实验中材料对红细胞、血小板、总蛋白的吸附均在10%以下。同时体外凝血酶原时间(PTs)及凝血酶时间(TTs)测定结果显示PS-PHE能延缓凝血速度。结论 PS-PHE对RF具有较高的吸附率及特异性并且表现出优良的血液相容性,在类风湿关节炎的临床治疗方面具有良好的应用前景。In this work we prepared an adsorbent （PS-PHE） aiming to remove rheumatoid factors and studied its performance.In the PS-PHE,the chloromethyl resin acted as carrier grafting with the linker of heparin,to which phenylalanine was connected.The adsorption rate of adsorbent was investigated by in vitro RF dynamic perfusion.We conducted in vitro elution experiment to measure the abscission rate of RF.Selectivity of adsorbent was evaluated by blood components before and after perfusion.The prothrombin time （PTs） and thrombin time （TTs） detection in vitro verify the blood compatibility of adsorbent.The adsorption equilibrium was reached in less than two hours,and 90％ of the maximum adsorption capacity was reached only under 1 hour,while the adsorption rate of IgA RF,IgG RF and IgM RF in serum was 45.21％ ± 1.80％ 、56.02％ ± 1.36％ 、52.40％ ± 2.01％ （n =5）.After elution,the abscission rate of IgA RF,IgG RF and IgM RF was 22.10％ ± 1.65％ 、19.23％ ± 1.06％ 、21.31％ ± 1.35％ （n =5）.After perfusion experiment,the variation rates of albumen and red blood cell were both less than 10％.The detection results of PTs and TTs showed PSPHE delayed the coagulation rate.In conclusion,PS-PHE has higher absorption rate to RF and good blood compatibility,showing good application prospects.

关 键 词：类风湿因子 免疫吸附剂 苯丙氨酸 

分 类 号：R318[医药卫生—生物医学工程]