高效液相色谱梯度洗脱法同时测定伤科跌打丸中4种有效成分的含量  

HPLC determination of 4 active components in Shangke Dieda pill

作　　者：陈海燕[1] 黄捷 

机构地区：[1]广西卫生职业技术学院,南宁530023 [2]广西食品药品检验所,南宁530021

出　　处：《中南药学》2014年第6期570-573,共4页Central South Pharmacy

摘　　要：目的采用高效液相梯度洗脱法测定伤科跌打丸中异鼠李素-3-O-新橙皮苷、香蒲新苷、升麻素苷和5-O-甲基维斯阿米醇苷的含量。方法 Hypersil C18色谱柱（4.6 mm×200 mm,5μm）;流速：0.9 mL·min-1;流动相A为乙腈,流动相B为0.05%磷酸溶液梯度洗脱;检测波长254 nm。结果异鼠李素-3-O-新橙皮苷、香蒲新苷、升麻素苷和5-O-甲基维斯阿米醇苷分别在0.031 2∽0.624 0μg（r=0.999 7）、0.023 8∽0.476 0μg（r=0.999 3）、0.015 8∽0.316 0μg（r=0.999 6）、0.010 2∽0.204 0μg（r=0.999 4）进样量与峰面积线性关系良好,平均加样回收率分别为98.4%、97.7%、98.5%、97.7%,RSD（n=6）分别为1.1%、0.82%、0.89%、0.79%。结论该含量测定方法结果准确、灵敏、重复性好。Objective To develop an HPLC method to determine the content of isorhamnetin-3-O-neohespeidoside, typhaneoside, prim-O-glucosylcimifugin and 4＇-O-β-D-glucosyl-5-O-methylvisammioside in Shangke Dieda pills. Methods Hypersil C18 column was used as the column, the flow rate was 0.9 mL·min-1. Mobile phase A consisted of acetonitrile, and the mobile phase B consisted of 0.05% phosphoric acid solution. The UV detection wavelength was 254 nm. Results There were a good linear relationship between the concentration of isorhamnetin-3-O-neohespeidoside, typhaneoside, prim-O-glucosylcimifugin, 4＇-O-β-D-glucosyl-5-O-methylvisammioside and peak area value when the concentrations of isorhamnetin-3-O-neohespeidoside, typhaneoside, prim-O-glucosylcimifugin and 4＇-O-β-D-glucosyl-5-O-methylvisammioside were 0.031 2-0.624 0 μg（r = 0.999 7）, 0.023 8-0.476 0 μg（r = 0.999 3）, 0.015 8-0.316 0 μg（r = 0.999 6）, and 0.010 2-0.204 0 μg（r = 0.999 4）. The average recoveris were 98.4%（RSD =1.1%）, 97.7%（RSD = 0.82%）, 98.5%（RSD = 0.89%） and 97.7%（RSD = 0.79%）. Conclusion The method is accurate, sensitive, reproducible and may be used in the determination of isorhamnetin-3-O-neohespeidoside, typhaneoside, prim-O-glucosylcimifugin and 4＇-O-β-D-glucosyl-5-O-methylvisammioside in Shangke Dieda pills.

关 键 词：伤科跌打丸 异鼠李素-3-O-新橙皮苷 香蒲新苷 升麻素苷 5-O-甲基维斯阿米醇苷 

分 类 号：R917[医药卫生—药物分析学]