高效液相色谱法测定夏天无胶囊中原阿片碱、盐酸巴马汀和比枯枯灵碱的含量  

HPLC determination of 3 effective ingredients in Xiatianwu capsules

作　　者：曾岗[1] 张洁[1] 

机构地区：[1]华容县人民医院药剂科,湖南岳阳414200

出　　处：《中南药学》2014年第6期588-591,共4页Central South Pharmacy

摘　　要：目的建立高效液相色谱法测定夏天无胶囊中原阿片碱、盐酸巴马汀、比枯枯灵碱的含量。方法 Agilent ZORBAX SB-C18色谱柱（250 mm×4.6 mm,4.6μm）,流动相A为乙腈,流动相B为醋酸（每100 mL 0.2%醋酸用1.5∽2 mL三乙胺调pH=5.1）,进行梯度洗脱,流速1.0 mL·min-1,检测波长282 nm,柱温：室温。结果原阿片碱、盐酸巴马汀和比枯枯灵碱分别在4.24∽101.8、2.89∽69.31和0.45∽10.84μg·mL-1与峰面积呈良好线性关系,低、中、高3个浓度平均加样回收率均〉95%,RSD均〈5%。结论该方法准确、快速、专属性强、灵敏度高,可用于夏天无胶囊中原阿片碱、盐酸巴马汀、比枯枯灵碱的含量测定。Objective To determine the content of protopine, palmatine hydrochloride and bicuculline in Xiatianwu capsules by HPLC. Methods HPLC was performed on an Agilent ZORBAX SB-C18 column（250 mm×4.6 mm, 4.6 μm） with a gradient of the following solvent mixtures： acetonitrile（A） and acetic acid（B）（dissolving 0.2 mL acetic acid in 100 mL water, adding 1.5-2 mL triethylamine to adjust pH to 5.1）. The flow rate was 1 mL·min-1, the detection wavelength was 282 nm, and the column temperature was room temperature. Results The linear relationship of protopine, palmatine hydrochloride, bicuculline were 4.24-101.8, 2.89-69.31, 0.45-10.84 μg·mL-1; the recoveries of low, middle, high concentration were more than 95% and the RSDs were less than 5%. Conclusion The method is rapid, accurate, and can be used for the determination of protopine, palmatine hydrochloride and bicuculline in Xiatianwu capsules.

关 键 词：夏天无胶囊 高效液相色谱法 原阿片碱 盐酸巴马汀 比枯枯灵碱 

分 类 号：R917[医药卫生—药物分析学]