Characterisation and Expression Analysis of Sox9 in the Multiocellated Racerunner, Eremias multiocellata  

作　　者：Ying XIN Huihui WANG Xiaolong TANG Songsong LU Weixin LI Yang ZHANG Shiwei LIANG Qiang CHEN 

机构地区：[1]Institute of Biochemistry and Molecular Biology, School of Life Science, Lanzhou University

出　　处：《Asian Herpetological Research》2014年第2期104-112,共9页亚洲两栖爬行动物研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 30670263)

摘　　要：Sox9 is an important member of Sox family which is involved in a variety of developmental processes including sex determination and gonadal differentiation. The cDNA of Sox9 from multiocellated racerunner E. multiocellata was cloned using reverse transcription-polymerase chain reaction （RT-PCR） and rapid amplification of cDNA ends （RACE）. The sequence contains a 1497 bp open reading frame, which encodes a 498 amino acid protein with a predicted molecular weight of 55.45 kDa. EmSox9 displays high similarity to those of reptiles, and shows an overall amino acid identity of 〉82%. We also investigated the tissue-specific expression of EmSox9 mRNA by realtime quantitative PCR. Sox9 mRNA is present in brain, heart, liver, kidney, gonads and muscle tissues of adult E. multiocellata, with the highest expression in brain and testis. The results indicate that Sox9 may play important roles in some tissues during E. multiocellata neural and gonadal development.Sox9 is an important member of Sox family which is involved in a variety of developmental processes including sex determination and gonadal differentiation. The cDNA of Sox9 from multiocellated racerunner E. multiocellata was cloned using reverse transcription-polymerase chain reaction （RT-PCR） and rapid amplification of cDNA ends （RACE）. The sequence contains a 1497 bp open reading frame, which encodes a 498 amino acid protein with a predicted molecular weight of 55.45 kDa. EmSox9 displays high similarity to those of reptiles, and shows an overall amino acid identity of 〉82%. We also investigated the tissue-specific expression of EmSox9 mRNA by realtime quantitative PCR. Sox9 mRNA is present in brain, heart, liver, kidney, gonads and muscle tissues of adult E. multiocellata, with the highest expression in brain and testis. The results indicate that Sox9 may play important roles in some tissues during E. multiocellata neural and gonadal development.

关 键 词：SOX9 HMG-BOX RACE Eremias multiocellata tissue specificity 

分 类 号：Q78[生物学—分子生物学]