干旱胁迫对芸豆种子萌发及生理特性的影响  

Effect of Drought Stress on Germination and Physiological Properties of Phaseolus coccineus Seeds

作　　者：王景伟[1,2] 金喜军[2] 杜文言[2] 史振声[1] 张玉先[2] 

机构地区：[1]沈阳农业大学,辽宁沈阳110866 [2]黑龙江八一农垦大学,黑龙江大庆163319

出　　处：《干旱区研究》2014年第4期734-738,共5页Arid Zone Research

基　　金：国家自然科学基金项目(204134008);农业部公益性行业(农业)科研专项(201303007);黑龙江八一农垦大学"大学生创新创业训练计划"项目

摘　　要：采用浓度水平分别为5%、10%、15%、20%和25%的聚乙二醇(PEG-6000)溶液,模拟干旱进行胁迫处理,研究干旱胁迫对芸豆种子萌发与幼苗生理特性的影响。结果表明:发芽进程、发芽率、发芽指数和活力指数等指标在轻度干旱胁迫时(5%)均无显著变化,随着干旱胁迫增加,萌发指标受到抑制作用而降低,胁迫越大,降幅越大;超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性则随干旱胁迫增加呈先增后降的趋势,SOD、CAT酶活性在10%浓度PEG处理时达最大,而POD酶活性在15%浓度时最大;丙二醛、脯氨酸含量的变化则呈单峰曲线趋势。The germination and physiological indices of Phaseolus coccineus seeds were investigated under artificial drought stress using 5%,10%,15%,20% and 25% polyethylene glycol solutions（ PEG- 6000）. The results showed that the effects of moderate drought stress on the germination process,germination rate,germination index and vigor index of P. coccineus seeds were not significant,these seed germination indexes were inhibited with the increase of drought stress,and the higher the drought stress,the most significant the decrease would be. SOD,CAT and POD activities were increased at first and then decreased with the increase of drought stress,the maximum values of SOD and CAT occurred at 10% PEG,and that of POD at 15% PEG. The curves of MDA and Pro content were unimodal.

关 键 词：干旱胁迫 芸豆(Phaseolus coccineus) 种子萌发 发芽率 保护酶活性 

分 类 号：S643.9[农业科学—蔬菜学]