小鼠嗜酸粒细胞性支气管炎模型与哮喘模型的蛋白组学差异表达分析  

Analysis of Comparative Proteomics of Eosinophilic Bronchitis Mouse Model and Asthma Mouse Model

作　　者：谢佳星[1] 张清玲[1] 陈莉延[1] 罗炜[1] 赖克方[1] 钟南山[1] 

机构地区：[1]广州医科大学附属第一医院,广东广州510120

出　　处：《医学临床研究》2014年第6期1049-1052,共4页Journal of Clinical Research

基　　金：国家自然科学基金项目资助（基金号81100028,81070019）

摘　　要：【目的】探讨小鼠嗜酸粒细胞性支气管炎模型（EB模型）与哮喘模型的蛋白组学差异表达。【方法】EB模型小鼠（实验组）、哮喘模型小鼠（哮喘组）及对照组小鼠各4只，取肺组织提取总蛋白质，采用基质辅助电离解析飞行时间质谱对蛋白质进行序列分析，并比较三组肺组织蛋白差异。【结果】双向电泳图像显示三组肺组织蛋白表达比较差异有显著性（P＜0．05），经质谱检测肽质量指纹谱与标准分子量、等电点对照分析鉴定出20个蛋白，分别为谷胱甘肽-S-转移酶 M1（GSTM1）、热休克蛋白 B1（HSPB1）等，其中 GSTM1在哮喘组表达下调、HSPB1在哮喘组表达上调。【结论】GSTM1、HSPB1等可能参与气道高反应性的发生机制。[Obj ective]To explore the differential expression of proteomics of eosinophilic bronchitis mouse model and asthma mouse model.[Methods]Four rats in each group including eosinophilic bronchitis model（EB model）（experimental group）,asthma model（asthma group）and control group.Total protein was extracted from lung tissue.Matrix-assisted laser desorption/ionization time of flight mass spectrometry was used for sequence a-nalysis of proteins.[Results]Two dimensional electrophoresis image showed that there was difference in the ex-pression of protein among 3 groups（P〈0.05）.Twenty proteins such as glutathione-S-transferase M1（GSTM1） and heat shock protein B1（HSPB1）were identified through the analysis of peptide mass fingerprint spectrum of mass spectrometry contrasted with standard molecular weight and isoelectric point.GSTM1 was down-regulated in asthma group,while HSPB1 was up-regulated in asthma group.[Conclusion]GSTM1 and HSPB1 may involve in the occurrence of airway hyperresponsiveness.

关 键 词：支气管炎 哮喘 疾病模型 动物 蛋白质组 小鼠 

分 类 号：R562.21[医药卫生—呼吸系统]