热修复中不同pH缓冲液对鸡睾丸组织免疫荧光结果的影响  

Impact on The Immunofluorescence Results of Testis in Different pH Buffers in Hot Fix

作　　者：赵辉[1] 陈建伟[1] 郭丽娜[1] 夏龙钢 张春香[1] 任有蛇[1] 

机构地区：[1]山西农业大学动物科技学院动物遗传育种与繁殖,山西太谷030801

出　　处：《黑龙江动物繁殖》2014年第4期15-18,共4页Heilongjiang journal of animal reproduction

基　　金：山西省农业科技攻关项目(20110311029)和(20130311025-2)资助

摘　　要：探讨热修复中不同pH缓冲液与不同修复温度对鸡睾丸组织免疫荧光结果的影响。选取可被抗体标记的阳性组织,采取不修复、胰蛋白酶修复、不同条件的热修复进行比较。结果表明,抗原修复是必要的,本试验中以缓冲液pH 7.0、97℃下的热修复效果最好。The objective of this study was to investigate the effects of different pH buffers and on immunofluorescence results in hot fix. Methods： Chose positive tissue which antibody compare the results in different conditions taken without repair, trypsin repair, hot fix conditions. best in this Results： Antigen retrieval was necessary, the hot fix effect in pH7.0 buffer at 97 ℃ was thebest in this experiment.

关 键 词：免疫组化 免疫荧光 抗原修复 

分 类 号：S831[农业科学—畜牧学]