巧克力培养基作为流感嗜血杆菌药敏培养基的可行性探讨  

Anlysis the feasibility of chocolate medium as culture medium of haemophilus influenzae

作　　者：罗宇鹏[1] 卢先雷[1] 张婷[1] 唐仕萍[2] 

机构地区：[1]成都市第五人民医院检验科,635000 [2]四川省简阳市中医院检验科,641400

出　　处：《检验医学与临床》2014年第A01期46-49,共4页Laboratory Medicine and Clinic

摘　　要：目的巧克力（CHO）培养基作为流感嗜血杆菌药敏培养基可行性探讨，判断巧克力培养基能否代替有效期短需现用现配的嗜血杆菌属试验培养基（HTM培养基）作为流感嗜血杆菌的药敏培养基，为各级医院减少工作量，节约检验成本。方法收集2012年5～12月临床标本分离的流感嗜血杆菌49株，分别用巧克力培养基和CLSI推荐培养基HTM培养基进行抗菌药物敏感试验，并以HTM培养基为参考标准，分析巧克力培养基上流感嗜血杆菌的抗菌药物敏感试验（以下简称药敏试验）结果，计算极重要误差、重要误差、次要误差，一致率。结果在所做的8种抗菌药物中，药物氨苄西林（AMP）、头孢呋辛（CXM）、复方磺胺甲恶唑（SXT）、环丙沙星（CIP）、头孢曲松（CRO）、亚胺培南（IPM）、阿奇霉素（AZM）未出现极重大误差，出现的重要误差均小于5％，这几种抗菌药物可以用巧克力培养基药敏试验结果取代HTM培养基药敏试验结果；但由于四环素（TCY）药敏试验出现了比较高的次要误差（32．7％），因此对于TCY的药敏试验不能用巧克力培养基代替HTM培养基。结论不推荐用巧克力培养基来代替HTM培养基来进行流感嗜血杆菌的体外药敏试验。Objective To study the feasibility of chocolate（CHO）culture-medium for haemophilus influenzae culture,which could be replace the HTM medium as drug susceptibility of haemophilus influenzae. Reducing the workload,saving the cost of inspection. Methods Collect clinical specimens of haemophilus influenzae 49 strains in May to December, 2012, With CHO culture-medium and HTM medium for antibacterial drug sensitive test, And HTM medium as the reference standard. Analysis of haemophilus influenzae antibacterial drug sensitive test results, calculate the very important error,error calculation,minor errors and concordance rate. Results The doing of 8 kinds of antimicrobial agents, AMP, CXM, SXT. CIP. CRO. IPM. AZM didn＇t appear very important error, the important error was 〈 5 %, The several kinds of antibiotics susceptibility test results could use CHO culture-medium instead of HTM mediums. TCY drug sensitive test appeared relatively high secondary error of 32.7 % ,So the drug sensitive test of TCY cann＇t replace HTM medium by chocolate culturemedium. Conclusion CHO culture-medium couldn＇t replace the HTM medium for haemophilus influenzae todrug sensitivity test in vitro.

关 键 词：流感嗜血杆菌 巧克力培养基 药敏 

分 类 号：R56[医药卫生—呼吸系统]