转化生长因子-β1基因多态性与乙型肝炎病毒慢性感染发展至肝细胞癌风险的相关研究  

Association between transforming growth factor-β1 gene polymorphism and risk of chronic hepaitis B virus infection progressing to hepatocellular carcinoma

作　　者：吴志强[1] 熊焰[1] 费青[1] 张志明[1] 伍仕敏[1] 

机构地区：[1]武汉市医疗救治中心检验科,武汉430023

出　　处：《国际检验医学杂志》2014年第13期1673-1675,共3页International Journal of Laboratory Medicine

摘　　要：目的：探讨转化生长因子-β1基因多态性与乙型肝炎病毒（HBV）慢性感染发展至肝细胞癌风险的关系。方法随机纳入120例 HBV 慢性感染患者（病例组）和100例与病例组性别、年龄相匹配的健康者（对照组），并将病例组分为非肝癌组和肝癌组。采用聚合酶链反应-限制性片段长度多态性分析法检测各组 TGF-β1基因多态性（T29C）并结合 TGF-β1 mRNA 水平进行比较分析。结果携带基因型 CC 发生肝癌的风险较携带基因型 TT 显著降低（OR ＝0．317，95％CI ＝0．110～0．913，P ＝0．033；OR＝284，95％CI ＝0．093～0．866，P ＝0．027）。携带等位基因 C 较等位基因 T 发生肝癌的风险显著降低（OR ＝0．570，95％CI ＝0．341～0．953，P ＝0．032；OR＝0．548，95％CI ＝0．320～0．936，P ＝0．028）。肝癌组中携带基因型 CC 的患者 TGF-β1 mRNA 水平较低。结论TGF-β1基因 T29C 多态性可能与 HBV 慢性感染进展至肝细胞癌风险有关。Objective To investigate the relationship between the polymorphism of transforming growth factor-β1 （TGF-β1 ） gene and risk of chronic hepatitis B virus（HBV）infection progressing to hepatocellular carcinoma（HCC）.Methods 120 patients with chronic HBV infection（case group）and 100 age-and sex-matched healthy individuals（healthy control group）were randomly enrolled to this study.The polymerase chain reaction-restriction fragment length polymorphism technique was adopted to detect the single nucleotide polymorphism of TGF-β1 gene（T29C）,and made the comparative analysis combined with TGF-β1 mRNA level. Results The risk of HCC occurrence in the patients carrying genotype CC was decreased than that in the patients carrying geno-type TT （OR=0.317,95%CI =0.110-0.913,P =0.033;OR=284,95%CI =0.093 -0.866,P =0.027）,the risk of HCC in pa-tients carrying allele C was significantly decreased compared with that in the patients carrying allele T（OR =0.570,95%CI =0.341 -0.953,P =0.032;OR=0.548,95%CI =0.320-0.936,P =0.028）.In the HCC group,the patients carrying genotype CC had the lower lever of TGF-β1 mRNA.Conclusion TGF-β1 gene polymorphism（T29C）may be related to the risk of chronic HBV infection progressing to HCC.

关 键 词：转化生长因子 多态现象 遗传 肝炎病毒 乙型 癌 肝细胞 

分 类 号：R512.62[医药卫生—内科学] R735.7[医药卫生—临床医学]