环氧化酶2抑制剂塞来昔布诱导NB4细胞凋亡的研究  

Study on effect of COX-2 selective inhibitor celecoxib on inducing apoptosis of NB4

作　　者：梅序桥[1] 吴阿阳[1] 郑源海[1] 柯靖兰 郑意 赵志坚 

机构地区：[1]福建医科大学附属漳州市医院检验科,福建漳州363000 [2]福建省漳州市血液中心,福建漳州363000

出　　处：《国际检验医学杂志》2014年第13期1676-1677,1680,共3页International Journal of Laboratory Medicine

摘　　要：目的研究环氧化酶2(COX-2)选择性抑制剂塞来昔布对髓性白血病细胞株NB4凋亡的影响,探讨塞来昔布诱导细胞凋亡的机制。方法 RT-PCR检测不同细胞系中COX-2mRNA表达,MTT法检测NB4细胞增殖抑制,DNA Ladder试验分析NB4细胞DNA片段化,流式细胞术检测NB4细胞Bcl-2蛋白的表达。结果与未加药处理组相比,不同浓度塞来昔布作用后DNA Ladder片段随浓度的增加而更为明显。不同用药组25μmol/L、50μmol/L、100μmol/L塞来昔布组Bcl-2蛋白表达率分别为(71.69±1.65)%、(34.51±2.53)%和(29.28±2.38)%,与空白对照组Bcl-2蛋白表达率为85.34±2.89%相比,50μmol/L、100μmol/L塞来昔布组Bcl-2蛋白明显下降(P<0.05)。结论 COX-2选择性抑制剂塞来昔布通过下调NB4细胞Bcl-2蛋白表达致诱导其凋亡。Objective To study the effects of COX-2 selective inhibitor celecoxib on the apoptosis of acute promyelocytic leuke-mia NB4 cell line,and to investigate its apoptosis mechanisms.Methods The expression of COX-2 mRNA in different cell lines was detected by reverse transcript polymerase chain reaction（RT-PCR）.After treatment of NB4 with different doses of celecoxib,the in-hibition of NB4 growth was assayed by MTT,and the DNA fragmentation was examined by the DNA ladder test.The level of Bcl-2 protein expression was assayed by the flow cytometry.Results As compared with the no-medication treatment group,the DNA ladder fragments became more and more obvious after the treatment by different doses of celecoxib.The expression rates of Bcl-2 protein in the different doses of celecoxib groups （25,50,100 μmol/L）were （71.69 ±1.65 ）%,（34.51 ±2.53）% and （29.28 ± 2.38）% respectively,compared with the Bcl-2 protein expression rate （85.34±2.89%）in the blank control group,the expression rate of Bcl-2 protein in different doses of celecoxib groups（50,100 μmol/L ）was significantly decreased（P 〈0.05 ）.Conclusion Celecoxib as COX-2 selective inhibitor could evidently induce the apoptosis of NB4 cells by down-regulating the expression of Bcl-2 protein in NB4 cells.

关 键 词：环氧水解酶类 细胞凋亡 逆转录聚合酶链反应 

分 类 号：R96[医药卫生—药理学]