抗菌肽人β防御素3融合糖类结合域对葡萄球菌的抑制作用  

Inhibition role of fusing antimicrobial peptides humanβ-defensin 3 and carbohydrate binding domain on staphylococcus

作　　者：黄洁雯[1,2] 郭晓奎[1,2] 李擎天[2] 

机构地区：[1]上海交通大学医学院免疫与病原生物学系,上海200025 [2]上海交通大学医学院检验系,上海200025

出　　处：《国际检验医学杂志》2014年第13期1678-1680,共3页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(81000708)

摘　　要：目的探讨抗菌肽人β防御素3融合糖类结合域(hBD3-CBD)对金黄色葡萄球菌N315、表皮葡萄球菌35984的抑制作用。方法以直接杀伤和分子生物学方法检测hBD3-CBD对金黄色葡萄球菌N315和表皮葡萄球菌35984菌株的抑制作用以及对细菌关键基因表达的影响。结果直接杀菌作用显示,抗菌肽hBD3以及hBD3-CBD对金黄色葡萄球菌N315、表皮葡萄球菌35984均有显著的抑制作用;hBD3-CBD的抑制作用强于hBD3;hBD3-CBD抑制作用的稳定性亦强于hBD3。在金黄色葡萄球菌N315、表皮葡萄球菌35984的关键基因表达检测中,hBD3-CBD对葡萄球菌的agr和mecA基因表达有显著抑制作用,还抑制表皮葡萄球菌icaA基因的表达和促进icaR基因表达,这说明hBD3-CBD能够抑制表皮葡萄球菌的生物膜形成。结论抗菌肽的融合策略对于改善抗菌肽的抑菌效能意义重大,并为其未来的应用带来更多希望。Objective To explore the inhibition of fusing antimicrobial peptides humanβ-defensin 3 and carbohydrate binding do-main on Staphylococcus aureus N315 and Staphylococcus epidermidis 35984.Methods The direct bactericidal test and other molec-ular biology methods were adopted to detect the inhibition role on Staphylococcus aureus strain N315 and Staphylococcus epidermi-dis strain 35984 and the influence on the key genes expression.Results The direct bactericidal test demonstrated that antimicrobial peptides hBD3 and hBD3-CBD had significantly inhibitory effects on staphylococcus aureus N315 and staphylococcus epidermidis 35984;the inhibitory effects of hBD3-CBD was stronger than that of hBD3;the stability of the inhibitory effects of hBD3-CBD also stronger than that of hBD3.In the key gene expression test,there were significant inhibitions on the agr and mecA gene expressions in Staphylococcus aureus N315 and Staphylococcus epidermidis 35984 by hBD3-CBD.At the same time,hBD3-CBD could inhibit the icaA gene expression and promote icaR gene expression in Staphylococcus epidermidis 35984,which indicated that hBD3-CBD could inhibit the biofilm formation of Staphylococcus epidermidis.Conclusion The fusion strategy of antimicrobial peptide has very important significance for improving the antibacterial efficacy of antimicrobial peptides and brings more hope in their future applications.

关 键 词：抗菌肽 葡萄球菌 金黄色 葡萄球菌 表皮 

分 类 号：R96[医药卫生—药理学]