流式细胞术检测血小板相关抗体在免疫性血小板减少症中的应用  

Application of flow cytommetry detection of platelet associated antibody in patients with immune thrombocytopenic pupura

作　　者：任娜[1] 邱广斌[1] 王成彬[1] 

机构地区：[1]中国人民解放军第二〇二医院检验科,辽宁沈阳110000

出　　处：《国际检验医学杂志》2014年第13期1689-1690,1693,共3页International Journal of Laboratory Medicine

基　　金：军队医学科研十二五重大专项(AWS11Z005-4);国家科技支撑计划(2013BAI17B05)

摘　　要：目的探讨流式细胞术检测血小板相关抗体在免疫性血小板减少症(ITP)的临床应用价值。方法 60例健康体检者、51例ITP患者(23例新诊断ITP患者、28例持续性ITP患者)和21例非ITP继发性血小板减少症患者的血小板相关抗体,PAIg的表达率及荧光强度,与血小板、巨核细胞数量进行相关性分析;分析持续性ITP经过治疗后与治疗前的PAIg表达率。结果ITP患者组IgG、IgA和IgM的荧光阳性百分率和平均荧光强度均明显高于健康对照组,差异有统计学意义(P<0.01)。与非免疫性血小板减少组比较,差异有统计学意义(P<0.05)。与自身免疫病比较,差异无统计学意义(P>0.05)。非免疫性血小板减少组与健康对照组比较,差异无统计学意义(P>0.05);自身免疫病组与健康对照组比较,差异有统计学意义P<0.01;在持续性ITP治疗患者中,完全反应(complete response,CR)组的PAIg与治疗前进行组间比较,差异有统计学意义(P<0.01)。有效(response,R)组的PAIg与治疗前组间比较,差异具有统计学意义(P<0.05)。结论流式细胞术检测PAIg可以用于ITP诊断、鉴别诊断和病情监测。Objective To investigate the value of the flow cytometry（FCM）for detecting platelet associated antibody in the di-agnosis of immune thrombocytopenia（ITP）.Methods Platelet associated antibody,expression rate and the fluorescence intensity of platelet-associated immunoglobulin（PAIg）were measured in 51 patients with ITP（23 cases of newly diagnosed ITP and 28 cases of persistent ITP）,21 patients with non-ITP and 60 healthy individuals.The correlation between the detection results with the platelet and megakaryocyte counts was performed;the expression rates of PAIg before and after treatment were compared.Results The fluorescence positive percentage and mean fluorescence intensity of IgG,Ig A and IgM in the patients group were significantly high-er than those in the control group with statistical difference（P 0.05）;the difference between the autoimmune disease group and the healthy control group had statistical significance（P 〈0.01 ）;in the persistent ITP group,PAIg in the complete response group had statistical difference between the groups before treament.PAIg in the response group also had statistical difference between the groups before treament （P 〈0.05）.Conclusion FCM for detecting PAIg can be used in the diagnosis,differential diagnosis and the disease condition monitoring of ITP.

关 键 词：流式细胞术 血小板 紫癜 血小板减少性 特发性 

分 类 号：R558.2[医药卫生—血液循环系统疾病]