化学发光免疫分析检测抗-HCV抗体与HCV-RNA的关联研究  

Association between HCV-RNA and chemiluminescence immunoassay for detecting anti-HCV antibody

作　　者：刘义庆[1] 邹建文[1] 田文君[1] 刘春梅[1] 王盛华[1] 王勇[1] 赵跃然[2] 张炳昌[1] 

机构地区：[1]山东大学附属省立医院检验科,山东济南250021 [2]山东大学附属省立医院中心实验室,山东济南250021

出　　处：《国际检验医学杂志》2014年第13期1749-1750,共2页International Journal of Laboratory Medicine

摘　　要：目的分析实时荧光定量PCR检测HCV-RNA载量与化学发光免疫分析(CLIA)检测抗-HCV抗体的相关性。方法抽取CLIA法抗-HCV检测有反应性标本587例,采用实时荧光定量PCR进一步检测HCV-RNA。结果荧光定量PCR检测HCV-RNA,阴性225例,阳性362例,阳性率为61.67%,且阳性标本比例与CLIA法S/CO比值呈正相关。结论 HCV-RNA阳性率与CLIA法S/CO比值呈正相关,可根据S/CO比值预测HCV-RNA结果。Objective To analyze the correlation of real-time fluorescent quantitation PCR（FQ-PCR）for detecting HCV-RNA loading and the chemiluminescence immunoassay（CLIA）for detecting anti-HCV antibody.Methods 587 samples of anti-HCV an-tibody positive detected by CLIA were furteher detected HCV-RNA by FQ-PCR.Results Among 587 samples of anti-HCV anti-body positive by the CLIA screening,225 samples were HCV-RNA negative and 362 samples were HCV-RNA positive detected by FQ-PCR,and the positive rate was 61 .67%,moreover,which was positively correlated with the S/CO ratio detected by CLIA.Con-clusion The positive rate of HCV-RNA is positively correlated with the S/CO ratio detected by CLIA.The result of HCV-RNA can be predicted according to the S/CO ratio.

关 键 词：肝炎抗体 丙型 化学发光测定法 聚合酶链反应 

分 类 号：R446.6[医药卫生—诊断学]