Pi-b、Pi-ta、Pikm和Pi54对水稻穗颈瘟的抗性评价  被引量:32

Evaluation of Resistance to Rice Panicle Blast with Resistant Genes Pi-b,Pi-ta,Pikm and Pi54

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作  者:范方军[1] 王芳权[1] 刘永峰[2] 王军[1] 朱金燕[1] 李文奇[1] 仲维功[1] 杨杰[1] 

机构地区:[1]江苏省农业科学院粮食作物研究所,江苏南京210014 [2]江苏省农业科学院植物保护研究所,江苏南京210014

出  处:《华北农学报》2014年第3期221-226,共6页Acta Agriculturae Boreali-Sinica

基  金:江苏省农业科技自主创新基金项目(CX[11]4020);国家科技支撑计划重大项目(2011BAD16B03)

摘  要:利用水稻稻瘟病抗病基因Pi-b、Pi-ta、Pikm和Pi54的功能标记检测2012年江苏省迟熟中粳稻预试64份品系,结合水稻穗颈瘟抗性鉴定,解析稻瘟病抗病基因Pi-b、Pi-ta、Pikm和Pi54在江苏省粳稻稻瘟病抗性育种中的作用。结果表明,在64份材料中含稻瘟病基因Pi-b的有49份,含Pi-ta的26份,含Pikm的19份,含Pi54的25份。64份品系的人工或病圃鉴定其抗性表现为2级中抗的有10份,3级感病的有43份,4级高感的有11份。64份品系中含有抗稻瘟病基因Pi-ta(26个新品系),或Pi-b、Pikm和Pi54组合(4个新品系),则该品系穗颈瘟抗性水平在2级中抗或3级感病,无4级高感,其他抗病基因组合其穗颈瘟抗性水平一般都存在4级高感。相关性分析表明,只有抗稻瘟病基因Pi-ta与穗颈瘟抗性存在显著相关性(r=0.344,P<0.01)。In this study,64 late maturity japonica test lines of Jiangsu Province were detected with the functional markers of rice blast resistance genes Pi-b,Pi-ta,Pikm and Pi54 in rice.Combined with the identification of rice panicle blast resistance,the role of blast resistance genes Pi-b,Pi-ta,Pikm and Pi54 for Japonica rice breeding with blast resistance in Jangsu Province has been analysized.There were 49 lines containing rice blast resistance gene Pib,26 lines containing Pi-ta,19 lines containing Pikm,25 lines containing Pi54 in 64 lines.Through artificial or disease nursery identification,the resistance performance into 2 levels( Moderate resistance) of disease resistance were10 lines,3 levels( Susceptible) were 43 lines,4 levels( High susceptible) were 11 lines.If new lines contain blast resistance genes Pi-ta( 26 new lines),or combined with Pi-b,Pikm and Pi54( 4 new lines),the levels of panicle blast resistance showed 2 levels( Moderate resistance) or 3 levels( Susceptible) and never 4 levels( High susceptible),while lines with other resistance gene combinations generally exhibited 4 grade levels of panicle blast resistance( High susceptible).A correlation showed that only the rice blast resistance gene Pi-ta has certain correlation with Panicle Blast Resistance( r = 0.344,P〈 0.01).

关 键 词:水稻 稻瘟病 功能标记 

分 类 号:S435.111.41[农业科学—农业昆虫与害虫防治]

 

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