利用UP-PCR、ISSR标记分析玉米弯孢叶斑病菌遗传多样性  

Genetic Diversity of Curvularia lunata by UP-PCR and ISSR Analysis

作　　者：王晓东[1] 高增贵[1] 姚远[1] 刘限[1] 苏家[1] 李婉莹[1] 

机构地区：[1]沈阳农业大学植物免疫研究所,辽宁沈阳110866

出　　处：《华北农学报》2014年第3期227-233,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家"十二五"技术支撑项目(2012BAD19B04;2013BAD07B03)

摘　　要：为明确辽宁省和安徽省玉米弯孢叶斑病菌的生理分化和遗传多样性及其近缘关系。利用UP-PCR、ISSR分子标记方法,对采集于辽宁省和安徽省30株玉米弯孢叶斑病菌进行了遗传多样性分析。从供试引物中筛选获得具有多态性好且稳定的UP-PCR引物4个、ISSR引物12个,分别扩增出27条谱带和76条谱带,多态性条带比率分别为77.78%和89.47%。聚类分析结果表明,UP-PCR阈值在0.662处菌株被分为7个类群,ISSR阈值在0.681处菌株被分为8个类群,玉米弯孢叶斑病菌存在丰富的遗传变异,与地理来源无明显相关性。从稳定性、可操作性和多态性水平来看,ISSR技术更适合于分析玉米弯孢叶斑病菌的遗传多样性。In order to understand the physiological differentiation,the genetic diversity and the relationship of Curvularia lunata in Liaoning Province and Anhui Province.Genetic diversity of 30 isolates of Curvularia lunata from the major corn producing areas of Liaoning Province and Anhui Province in China was analyzed by UP-PCR（ Universally primed PCR） and ISSR（ Internal simple sequence repeat）.The results showed that 4 primers of UPPCR,12 primers of ISSR Genetic 27 and 76 polymorphic bands,accounted for 77.78% and 89.47% of total bands respectively.These molecular markers revealed the genetic diversity of the strains tested,and there was no significant correlation between variation and region.The correlation between UP-PCR and ISSR,the dendrogram based on UP-CR results revealed that the 30 isolates were clustered into 7 groups at the threshold of genetic similar coefficient of 0.662,while ISSR were clustered into 8 groups at the threshold of genetic similar coefficient of 0.681.The ISSR technique was suitable for the genetic diversity analysis of C.lunata in view of the level of stability,operability and polymorphism.

关 键 词：玉米 弯孢叶斑病菌 UP-PCR ISSR 遗传多样性 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]