延边地区长柱金丝桃中4种酮类成分的含量测定  

Determination of Four flavonoids in Hypericum ascyron L.of Yanbian area

作　　者：姜英子[1] 王科斯 金慧颖[2] 安仁波[1,3] 

机构地区：[1]延边大学药学院,吉林延吉133002 [2]延边第二人民医院,吉林延吉133001 [3]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《延边大学学报（自然科学版）》2014年第2期182-185,共4页Journal of Yanbian University（Natural Science Edition）

基　　金：教育部留学回国人员科研启动基金资助项目(2008890);吉林省科技发展计划项目(200705427)

摘　　要：为长柱金丝桃（Hypericum ascyron L.）质量标准的研究提供参考,在色谱柱为Diamonsil C18（250mm×4.6mm,5＂m）、流动相为甲醇乙腈0.6%磷酸水溶液、线性度（R2）高于0.999 0、回收率为92.15%～108.37%、相对标准偏差为1.57%～1.91%的条件下,使用高效液相色谱法同时测定了延边地区长柱金丝桃中芦丁、金丝桃苷、槲皮素和山奈酚4种成分的含量.实验结果表明：长柱金丝桃中芦丁、金丝桃苷、槲皮素和山奈酚的含量随区域有较大差异;本文方法准确、灵敏、可靠.To provide references for quality standard of Hypericum ascyron L.,HPLC method was established for simultaneous determination of rutin,hyperoside,quercetin and kaempferol in Hypericum ascyron L.of Yanbian area.Chromatographic separation was employed on Diamonsil C18column（250mm×4.6mm,5＂m）with gradient elution of methanol-acetonitrile-0.6% phosphoric acid as mobile phase.Calibration curves of standard components show excellent linearity with R20.999 0and the recovery tests are found to range from92.15%to 108.37% with relative standard deviation values 1.57% 1.91%.The contents of flavonoids were diferent obviously in samples from diferent regions.This method is accurate,sensitive and reliable.

关 键 词：延边地区 长柱金丝桃 芦丁 金丝桃苷 槲皮素 山奈酚 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]