HPLC法测定患者丙戊酸钠血药浓度  

Determination of the concentration of sodium valproate in patients' serum by HPLC

作　　者：段自皞[1] 王法财[1] 沈炳香[1] 聂松柳[1] 

机构地区：[1]安徽医科大学六安附属医院药学部,六安237005

出　　处：《海峡药学》2014年第6期152-154,共3页Strait Pharmaceutical Journal

摘　　要：目的建立反相高效液相色谱法测定人血清中丙戊酸钠（VPA）血药浓度的方法学。方法血清酸化后用环戊烷提取,选择环己烷羧酸为内标,以α-溴苯乙酮为衍生化试剂,采用Symmetry C18（250mm×4.6mm,5μm）分析柱,流动相为乙腈-水（75∶25）,检测波长为248nm,流速1mL·min-1,柱温35℃。用高效液相色谱法测定丙戊酸钠血药浓度。结果内标环己烷羧酸和VPA的保留时间分别为10.0min,15.0min,分离效果良好,丙戊酸钠（VPA）血药浓度在6.25～200μg·mL-1范围内呈现良好的线性关系（R=0.9982）,平均回收率98.2%,日间RSD〈5%。结论本法快速、简便、准确,适合临床常规监测需要。OBJECTIVE To determine sodium valproate in patients＇ serum by high performance liquid chromatography（ HPLC）. METHODS Serum sample（ 100μL） was extracted with n-pentane after acidification. Cyclohexanecarboxylic acid and α-bromoacetophenone were selected as an internal standard and derivative reagent respectively. The analytical column was Symmetry C18（ 250mm ×4.6mm,5μm） with the temperature at 35℃. The detection wavelength was 248nm and the flow rate was 0. 8mL·min- 1. The mobile phase consisted of acetonitrile-water（ 75∶25）. The blood concentration of sodium valproate was determined by HPLC. RESULTS The retention time of internal standard and VPA were 10. 0min and 15. 0mim. The linear range of sodium valproate was 6. 25～ 200mg·L- 1,the mean recovery was 98.2%. The relative standard deviation（ RSD） of inter-day for sodium valproate was less than 5%. CONCLUSION The method is rapid,accurate,sensitive and suitable for clinica1 routine monitoring.

关 键 词：丙戊酸钠 血药浓度 高效液相色谱法 柱前衍生化 

分 类 号：R969.4[医药卫生—药理学]