骨髓间充质干细胞条件培养液对急性百草枯中毒肺损伤的影响  

Effect of bone marrow mesenchymal stem cells conditioned medium on rats with acute paraquat poisoning lung injury

作　　者：熊剑飞[1] 朱长清[1] 张柯基[1] 吕利雄[1] 归茜[1] 陆晓晔[1] 

机构地区：[1]上海交通大学医学院附属仁济医院急诊科,上海200127

出　　处：《临床肺科杂志》2014年第8期1371-1374,共4页Journal of Clinical Pulmonary Medicine

摘　　要：目的通过将骨髓间充质干细胞(BMSCs)条件培养液注入到急性百草枯中毒大鼠体内,观察其对急性百草枯中毒肺损伤的影响。方法选4周龄SPF级雄性SD大鼠,原代培养BMSCs,传至第3代的BMSCs无血清培养24 h,收集的培养基即为BMSCs条件培养液。将30只SPF级雄性大鼠随机分为3组:百草枯组、BMSCs条件培养液治疗组(治疗组)、正常对照组。将BMSCs条件培养液通过尾静脉注入各组大鼠体内,观察各组大鼠肺组织肺泡炎评分、肺组织α-SMA表达水平和各组大鼠血浆中TNF-α、IL-1β的水平变化。结果与百草枯组相比,治疗组肺泡炎症反应较轻,肺组织α-SMA表达水平较低(P<0.05),血浆中TNF-α、IL-1β的含量较低(P<0.05)。结论 BMSCs条件培养液可减缓急性百草枯中毒引起的肺损伤。Objective To investigate the effect of bone marrow mesenchymal stem cells（ BMSCs） conditioned medium on rats with paraquat-induced acute lung injury. Methods BMSCs were isolated from male SD rats.BMSCs conditioned medium was obtained after BMSCs serum-free cultured for 24 hours and injected into recipient SD rats. 30 male recipient SD rats were randomly divided into three groups： the PQ group,the BMSCs conditioned medium treatment group,and the control group. Lung tissues were observed by HE staining and score of alveolitis was calculated. At the same time,the expression of α-smooth muscle actin in lung tissues was assessed. The levels of tumor necrosis factor-α（ TNF-α） and interleukin-1β（ IL-1β） in plasma were measured. Results Compared with the PQ group,the alveolar inflammation was lighter in the treatment group. The score of alveolitis was reduced in the treatment group（ P 0. 05）. The expression of α-smooth muscle actin in lung tissue of the treatment group was lower than that of the PQ group（ P〈0. 05）. The levels of TNF-α and IL-1β in plasma decreased markedly in the treatment group（ P〈 0. 01）. Conclusion BMSCs conditioned medium can alleviate paraquat-induced acute lung injury in rats.

关 键 词：骨髓间充质干细胞 条件培养液 百草枯 中毒 肺损伤 

分 类 号：R563.8[医药卫生—呼吸系统] R595.4[医药卫生—内科学]