小檗碱对金黄色葡萄球菌诱导的ECV-304细胞凋亡相关基因的影响  

Effects of berberine on apoptosis related genes of ECV-304 cells induced by staphylococcus aureus

作　　者：裴德翠 熊传银 毕爱芬 胡汉斌 

机构地区：[1]广州市中西医结合医院检验科

出　　处：《药物与人》2014年第8期3-4,共2页Medicine & People

基　　金：广东省中医药局项目(编号:20121029)

摘　　要：目的：研究小檗碱对金黄色葡萄球菌（S.aureus）诱导ECV-304细胞内凋亡相关基因Bcl-2、Bax和Caspase-3表达的调节作用。方法：小檗碱（Berberine,BBR）预处理ECV-304细胞2h后,用S.aureus感染细胞,采用半定量RT-PCR及Western-blot法检测金黄色葡萄球菌感染后ECV-304细胞内Bcl-2、Bax和Caspase-3的mRNA及蛋白表达水平。结果：小檗碱预处理ECV-304后再感染S.aureus可显著性增加凋亡相关细胞因子Bcl-2mRNA水平及蛋白表达量,而Bax和Caspase-3表达下调。结论：小檗碱可以调节S.aureus诱导的ECV-304细胞内凋亡相关基因的表达,抑制ECV-304细胞凋亡。Objective：To study the protection of berberine against ECV- 304 apoptosis induced by Staphylococcus aureus（S.aureus） via regulating Bcl-2,Bax and caspase-3 expression.Methods：ECV-304 were pretreated with 128μg/m LBBR for 2h,then Saureus was added.The expression levels of apoptosis related genes were detected with semi-quantityRT- PCR and Western- blot.Results：Pretreatment of BBR helped to survive in Saureus- induced ECV- 304.BBR significantly increased the expression levels of Bcl-2 and decreased that of Bax and Caspase-3 after staphylococcus aureus infection.Conclusion：Berberine can regulate the expression of apoptosis related genes of ECV-304 cells induced by S.aureus.

关 键 词：小檗碱 金黄色葡萄球菌 ECV-304 凋亡 

分 类 号：R856.2[医药卫生—航空、航天与航海医学]