丹参酮ⅡA抑制髓系白血病细胞株增殖及诱导凋亡的研究  

Effects of Tanshinone ⅡA on proliferation and apoptosis of myeloblastic leukemia cell lines

作　　者：李慧[1] 刘美[2] 黎红佳[2] 黎伟[2] 谢文瑾 

机构地区：[1]四川省医学科学院四川省人民医院血液科,成都610072 [2] 四川大学华西药学院 [3] 福建省福清核电有限公司保健物理处

出　　处：《白血病．淋巴瘤》2014年第6期334-338,共5页Journal of Leukemia & Lymphoma

基　　金：四川省卫生厅科学研究项目（130209）

摘　　要：目的 探讨丹参酮ⅡA（TanⅡA）对髓系白血病细胞株NB4、K562、THP-1增殖抑制作用及促凋亡作用.方法 将不同浓度TanⅡA与NB4、K562、THP-1细胞共培养24、48、72 h,以柔红霉素为阳性对照,采用四甲基偶氮唑蓝（MTT）法检测TanⅡA对白血病细胞株的增殖抑制作用,AnnexinV-FITC/PI流式细胞术检测细胞凋亡和细胞周期,紫外分光光度法检测Caspase-3蛋白表达.结果 TanⅡA作用24、48、72 h对NB4细胞的IC50值分别为24.11、9.60、7.28 μmol/L,对K562细胞IC50值分别为31.75、11.88、6.81 μmol/L,对THP-1细胞IC50值分别为111.10、32.82、11.82 μmol/L.流式细胞术检测结果显示：与空白对照组相比,TanⅡA与各白血病细胞株作用48 h后,细胞凋亡明显增加,G1期细胞数增加,Caspase-3蛋白表达显著升高（P＜0.05）.结论 TanⅡA对白血病细胞具有增殖抑制与促凋亡作用,其作用强度从高到低依次为NB4、K562、THP-1细胞.其抗癌作用机制可能与阻滞细胞于G1期,上调Caspase-3表达有关.Objective To investigate effects of Tanshinone Ⅱ A （Tan Ⅱ） on proliferation and apoptosis of myeloblastic leukemia cell lines.Methods NB4,K562 and THP-1 cells were incubated with TanⅡA for 24,48 and 72 hours.Ddaunorubicin was used as a positive control.Cell proliferation was monitored by MTT assay.Cell apoptosis and cell cycle were determined by Annexin Ⅴ-FITC/PI flow cytometry.Expression of Caspase-3 was quantified by spectrophotometry.Results After incubation with various leukemia cells for 24,48 and 72 hours,Tan Ⅱ inhibited proliferation of NB4 cells with IC50 of 24.11,9.60 and 7.28 μmol/L,inhibited K562 cells with IC50 of 31.75,11.88 and 6.81 μmol/L and inhibited THP-1 cells with IC50 of 111.10,32.82 and 11.82,respectively.After treatment with Tan Ⅱ for 48 hours,cell apoptosis,the number of G1 phase cells and the expression of Caspase-3 in all three leukemia cell lines were increased significantly comparing with the blank control group （P 〈 0.05）.Conclusions Tan Ⅱ A has proliferation inhibitory effect on myeloblastic leukemia cell lines by the order of effect NB4〉K562〉THP-1.Tan ⅡA displays anti-leukemia activity possibly through arresting leukemia cells in G1 phase and inducing apoptosis by increasing Caspase-3 expression.

关 键 词：白血病 丹参酮ⅡA 细胞凋亡 细胞周期 半胱氨酸天冬氨酸蛋白酶3 

分 类 号：R285.5[医药卫生—中药学]