ACE2对大鼠心肌梗死后心肌重构的影响  

Effect of ACE2 on myocardial remodeling after myocardial infarction in rat

作　　者：崔贞玉[1,2] 韩素霞[1] 董晓光[1] 冯磊[1] 常建梅[1] 郭李平[1] 

机构地区：[1]新疆医科大学第五附属医院心血管内科,乌鲁木齐830011 [2]安阳市人民医院心内科,河南安阳455000

出　　处：《实用药物与临床》2014年第7期811-815,共5页Practical Pharmacy and Clinical Remedies

基　　金：国家自然科学基金(81060023)

摘　　要：目的探讨大鼠心肌梗死(MI)后心肌组织中AngⅡ的表达与心肌重构的关系,进一步阐明ACE2对大鼠MI后心肌重构的作用机制。方法将32只健康雄性SD大鼠随机分为假手术组(n=8)、MI组(n=8)、氯沙坦小剂量组[MI+10 mg/(kg·d),n=8]和大剂量组[MI+20 mg/(kg·d),n=8],随后将MI组和氯沙坦组大鼠建立心尖部心梗模型。术中检测梗死前后心电图变化情况。术后4周取大鼠心尖部心肌组织,HE染色观察心肌组织病理改变情况,RT-PCR检测心肌内AngⅡ和ACE2的mRNA表达量。结果 HE染色结果显示,MI组与假手术组相比,大鼠心肌细胞肿胀、变形、破裂增多,MI组心肌内AngⅡ的mRNA表达量显著高于假手术组,给予氯沙坦后,表达量呈剂量依赖性下降。MI组心肌内AngⅡ的mRNA表达量显著高于假手术组,给予氯沙坦后,表达量呈剂量依赖性下降。MI组心肌内ACE2的mRNA表达量略高于假手术组,氯沙坦组继续升高,呈剂量依赖性。结论氯沙坦组大鼠ACE2表达量增加,进而通过抑制心肌组织内AngⅡ表达来改善MI后的心室重构。Objective To investigate the effect of ACE2 on myocardial remodeling after myocardial infarction.Methods 32 SD rats were randomly divided into 4 group,sham-operated group（n = 8）,myocardial infarction group（MI,n = 8）,losartan small-dose group[MI ＋ 10 mg /（kg·d） for 4 weeks,n = 8] and losartan high-dose group[MI ＋20 mg/（kg·d） for 4 weeks,n =8].Then established the myocardial infarction models,and traced the electrocardiogram in intraoperative.After 4 weeks,observing the pathological changes in myocardium by HE staining,the mRNA expression of AngⅡ and ACE2 in myocardium were detected by RT-PCR.Results Myocyte swelling,deformation and fracture were increased significantly in MI group compared with sham-operated group and were alleviated in losartan group in a dose-dependent.The mRNA expression of AngⅡ significantly upregulated in MI group than in sham-operated group,and downregulated in losartan group in a dose-dependent.The mRNA expressions of ACE2 were slightly higher in MI group than in sham-operated group and further increased in losartan group in a dose-dependent.Conclusion ACE2 can reverse myocardial remodelling by decreasing the expression of AngⅡ in losartan group.

关 键 词：心肌梗死 AngⅡ 心肌重构 氯沙坦 ACE2 

分 类 号：R965[医药卫生—药理学]