HPLC法测定复方氯霉素醇溶液中氯霉素和水杨酸的含量  

Determination of chloramphenicol and salicylic acid in compound chloramphenicol alcoholic solution by HPLC

作　　者：吴海伟[1] 贾燕花[1] 王晓青[1] 高磊[1] 薛谟斐 刘皈阳[1] 

机构地区：[1]中国人民解放军总医院第一附属医院,北京100048

出　　处：《实用药物与临床》2014年第7期878-881,共4页Practical Pharmacy and Clinical Remedies

基　　金：军队"十二五"军队医疗机构制剂标准提高专项课题(13ZJZ20)

摘　　要：目的建立高效液相色谱法同时测定复方氯霉素醇溶液中氯霉素和水杨酸的含量。方法色谱柱：Eclips XDB-C18（4.6 mm×250 mm,5μm）;流动相：1%冰醋酸-甲醇（60∶40）;流速：1.0 mL/min,进样量10μL;检测波长为293 nm。结果氯霉素、水杨酸线性范围均为6-160μg/mL（r=1）;平均回收率分别为99.45%（RSD=0.46%,n=6）,100.32%（RSD=0.97%,n=6）。结论该方法简便、准确、可靠,可同时测定复方氯霉素醇溶液中氯霉素、水杨酸的含量。Objective To develop a method for the simultaneous determination of chloramphenicol and salicylic acid in compound chloramphenicol alcoholic solution by HPLC.Methods HPLC was used for quantitative analysis.The Eclips XDB-C18（4.6 mm ×250 mm,5 μm） was used,and the mobile phase composed of 1%acetic acid solution-methanol（60∶ 40）.The flow rate was 1.0 mL /min.The detection wavelength was at 293 nm and the injection volume was 10 μL.Results The linear ranges of chloramphenicol and salicylic acid were 60 ~ 160 μg /mL（r = 1）,with the average recoveries of 99.45% and 100.32%,RSD of 0.46% and 0.97% respectively.Conclusion The method is simple,accurate with good reliability.It is suitable for the determination of chloramphenicol and salicylic acid simultaneously in compound chloramphenicol alcoholic solution.

关 键 词：HPLC法 氯霉素 水杨酸 含量测定 

分 类 号：R927[医药卫生—药学] O657.72[理学—分析化学]