雷公藤多苷对糖尿病肾病大鼠肾脏结缔组织生长因子表达的影响  

Effect of Tripterygium wilfordii polyglycosidum on connective tissue growth factor in liver in diabetic nephropathy rats

作　　者：刘国玲[1] 芦琨[1] 尤丽菊[1] 郭辉[1] 李宜川[1] 

机构地区：[1]商丘医学高等专科学校生物化学与分子生物学教研室,河南商丘476100

出　　处：《中国临床药理学杂志》2014年第7期603-606,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河南省教育厅自然科学研究计划基金资助项目(2011C310003)

摘　　要：目的观察雷公藤多苷(TWP)对糖尿病肾病(DN)大鼠肾脏结缔组织生长因子(CTGF)表达和排泄的影响。方法用腹腔注射链脲佐菌素的方法建立糖尿病大鼠模型。动物随机分为正常组、模型组、3个剂量(45,90,180mg·kg-1)TWP组;用药8周末,检测大鼠血糖(BG)、体重(BW)、肾重(KW)、肾重/体重(KW/BW)B、24 h尿微量蛋白清除率(UAER)、血尿素氮(BUN)和肌酐清除率(Ccr);HE染色观察大鼠肾组织病理学变化;免疫组化检测肾皮质CTGF蛋白表达。结果与模型组相比,TWP能明显改善DN大鼠一般情况,高剂量TWP组空腹BG明显降低(P<0.01),中、高剂量TWP组能使BW明显增加(P<0.01),同时使KW、KW/BW、24hUAER、BUN及Ccr也明显降低(P<0.01),使肾组织着色程度明显减轻,使CTGF表达显著下降(P<0.01),并使肾小球肥大、系膜细胞增生及肾小球变形病变均明显减轻。结论 TWP能明显改善肾脏功能,且与剂量相关,其机制可能与抑制肾脏CTGF表达有关。Objective To investigate the effect of Tripterygium wilfordii polyglycosidum （ TWP ） on the expression and excretion of connective tissue growth factor （ CTGF ）.Methods The diabetes mellitus Sprague Dawley rats was induced by streptozotocin .The diabetic rats were random-ly divided in to five groups：normal group ,diabetic model group and three doses （45,90,180 mg · kg -1 ） of TWP groups.Blood glucose （ BG）, body weight （ BW） , kidney weight （ KW） , KW/BW, 24 hours urinary al-bumin excretion rate （ UAER ） , blood urea nitrogen （ BUN ） , creatinine clearance （ Ccr） were measured at the end of the 8th week.The patholo-gy changing of the rat kidney was observed by HE staining , and the ex-pression of CTGF was determined by immunohistochemical assay.Re-sults Compared with model group , TWP can significantly improve the general situation of DN rats.TWP （180 mg· kg -1 ） can significantly de-creased fasting BG （ P〈0.01 ）.TWP （ 90 and 180 mg· kg -1 ） can sig-nificantly increase BW of rats （ P〈0.01 ） , and TWP （ 90 and 180 mg · kg -1 ） also can significantly reduce KD , KW/BW, 24hUAER, BUN （ P〈0.01 ）.And the coloring of the renal tissue mitigate signifi-cantly, the expression of CTGF significantly decreased in TWP （90 and 180 mg· kg -1 ） groups; glomerular hypertrophy , mesangial cellproliferation and glomerular lesions all significantly reduce.Conclusion The TWP can obviously improve the renal function, and dose-related, and its mechanism may be correlated with inhibition on expression of CTGF in kindey.

关 键 词：雷公藤多苷 糖尿病肾病 结缔组织生长因子 肾小球 免疫组化法 

分 类 号：R965.1[医药卫生—药理学]